两种绣线菊低温锻炼与脱锻炼处理对蛋白质表达的影响  

Protein expression of two Spiraea species under effects of cold acclimation and de-acclimation

作　　者：刘慧民[1] 闫永庆[1] 车艳双[1] 冯楠楠[1] 张超[1] 樊超[1] 车代弟[1] 

机构地区：[1]东北农业大学园艺学院园艺系,哈尔滨150030

出　　处：《生态学杂志》2009年第7期1330-1334,共5页Chinese Journal of Ecology

基　　金：黑龙江省教育厅科研项目(10551027);哈尔滨市科技创新人才专项(2006RFQXN012);东北农业大学创新团队资助项目(CXZ004-3)

摘　　要：通过2维双向电泳法,研究抗寒能力与光合能力不同的金山绣线菊与华北绣线菊,低温锻炼处理对蛋白质表达所产生的影响。结果表明,低温锻炼处理对2种绣线菊蛋白质表达的影响显著不同,在低温锻炼与脱锻炼处理中,金山绣线菊产生低温诱导特异蛋白,华北绣线菊没有低温诱导特异蛋白表达;蛋白质双向电泳图谱分析表明,华北绣线菊与金山绣线菊经过低温锻炼后发生蛋白质差异表达,经质谱鉴定其中的3种显著差异表达的蛋白质分别为2种未知新蛋白,2种未知蛋白的部分氨基酸序列分别为VSHLAGFSSNNPK、LKADKPTLLSEAK;DPNDHPNPFTVK、DGNGFFLYLLDPDSSK、NGDGMFLYLLDGLESK,另一种差异蛋白为抗坏血酸过氧化物酶;低温锻炼处理使绣线菊中与光合作用密切相关的蛋白质抗坏血酸过氧化物酶发生了显著的变化。By the method of two-dimensional electrophoresis, this paper studied the effects of cold acclimation and de-acclimation on the protein expression of two Spiraea species with different cold resistance and photosynthesis capability. During cold acclimation and de-acclimation, S. bumalda ＇ Goldmound＇ produced cold acclimation-induced protein, while S. fritschiana did not. Two-dimensional electrophoresis indicated that after cold acclimation, S. fiitschiana and S. bumalda ＇ Goldmound＇ had different protein expression. Through MS identification, there were three kinds of protein conspicuously expressed, two of which were unknown new proteins, their partial amino acid sequences being VSHLAGFSSNNPK and LKADKPTLLSEAK, and DPNDH-PNPFTVK, DGNGFFLYLLDPDSSK, and NGDGMFLYLLDGLESK, respectively, and the other was ascorbate peroxidase, an enzyme closely related to photosynthesis. Cold acclimation made the ascorbate peroxidase in the two Spiraea species changed significantly.

关 键 词：绣线菊 低温锻炼 二维双向电泳 特异蛋白 

分 类 号：S685.99[农业科学—观赏园艺]