饲料中不同维生素C含量对长吻的影响  

EFFECTS OF DIFFERENT DIETARY VITAMIN C LEVELS ON CHINESE LONGSNOUT CATFISH(LEIOCASSIS LONGIROSTRIS GNTHER)

作　　者：刘海燕[1,2] 雷武[1] 朱晓鸣[1] 杨云霞[1] 韩冬[1] 薛敏[2] 解绶启[1] 

机构地区：[1]中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,武汉430072 [2]中国农业科学院饲料研究所,北京100081

出　　处：《水生生物学报》2009年第4期682-689,共8页Acta Hydrobiologica Sinica

基　　金：湖北省水产重大专项(2006AA203A01);十一五国家科技支撑计划(2006BAD03B01);淡水生态与生物技术国家重点实验室开放基金(2009FB09)资助

摘　　要：用维生素C含量为38、364和630mg/kg的三种饲料饲喂初体重为(23.19±0.58)g的长吻8个月,实验结果表明,38mg/kgVC饲料组饲喂的长吻表现出了典型的VC缺乏症状:体色发黑,脊椎侧弯,鳍边由开始的萎缩直到腐烂、贫血、特定生长率显著降低(p<0.05)。并且38mg/kgVC饲料组长吻的生理指标也受到影响:血清溶菌酶活性显著下降(p<0.05),肝脏的SOD活性及MDA含量均显著升高(p<0.05),血清皮质醇没有显著差异(p>0.05),但各处理组长吻肝脏中均未检测到诱导型HSP70的存在。因此,相对于皮质醇和HSP70,血清溶菌酶、肝脏SOD活性、肝脏MDA含量以及血液理化指标(血红细胞数及血红蛋白)更能反映出VC缺乏下长吻的生理状态。Vitamin C is an essential micro-nutrient for most fish and plays an important role in immunity and physiology. The deficiency of dietary vitamin C can result in the damage on fish. The study on the deficiency symptom and the sensitive indicator of vitamin C could provide useful information for fish feed industry. This experiment was conducted to investigate the effects of dietary Vitamin C levels on growth performance, immune and physiological responses in Chinese longsnout catfish （Leiocassis longirostris Giinther） , which is an high value aquaculture fish in China. Three practical diets containing 38, 364 and 630mg/kg vitamin C were fed to Chinese longsnout catfish （23.19 ±0.58g） for 8 months. Vitamin C was used in the form of L-ascorbyl-2-polyphosphate. The results indicated that the Chinese longsnout catfish in 38mg/kg vitamin C groups showed typical vitamin C deficiency symptom： skin color turning darker, spinal deformities and scoliosis, erosion of fins, anaemia. SGR and the serum lysozyme activities decreased significantly （p 〈 0.05 ） , the hepatic SOD activities and MDA contents increased significantly （p 〈 0.05）. There was no significant difference in serum cortisol concentrations （p 〉 0.05）. The inductive HSP70 in liver was not detected by western-blotting in all groups. In conclusion, serum cortisol and hepatic HSP70 were not the sensitive indicators for vitamin C deficiency symptom, but the serum lysozyme, hepatic SOD activity, hepatic MDA content and the haematological index （ red blood cell number and hemoglobin content） were the more reliable makers to indicate the physiological status in Chinese longsnout catfish.

关 键 词：维生素C 维生素C缺乏症 长吻 

分 类 号：S963[农业科学—水产养殖]