Stem cells modified by brain-derived neurotrophic factor to promote stem cells differentiation into neurons and enhance neuromotor function after brain injury  

作　　者：张赛 刘晓智 刘振林 王延民 胡群亮 马铁柱 孙世中 

机构地区：[1]Department of Neurosurgery, Affiliated Hospital of Medical College of Chinese People's Armed Police Force, Tianjin 300162, China

出　　处：《Chinese Journal of Traumatology》2009年第4期195-199,共5页中华创伤杂志（英文版）

基　　金：This study was supported by the Project of Science and Technology Funds of Tianjin （No.06YFSZSF01200） and the National Natural Science Foundation of China （No. 30872668）

摘　　要：Objective： To promote stem cells differentiation into neurons and enhance neuromotor function after brain injury through brain-derived neurotrophic factor （BDNF） induction. Methods： Recombinant adenovirus vector was applied to the transfection of BDNF into human-derived umbilical cord mesenchymal stem cells （UCMSCs）. Enzyme linked immunosorbent assay （ELISA） was used to determine the secretion phase of BDNF. The brain injury model of athymic mice induced by hydraulic pressure percussion was established for transplantation of stem cells into the edge of injury site. Nerve function scores were obtained, and the expression level of transfected and non-transfected BDNF, proportion of neuron specific enolase （NSE） and glial fibrillary acidic protein （GFAP）, and the number of apoptosis cells were compared respectively. Results： The BDNF expression achieved its stabilization at a high level 72 hours after gene transfection. The mouse obtained a better score of nerve function, and the proportion of the NSE-positive cells increased significantly （P〈0.05）, but GFAP-positive cells decreased in BDNF- UCMSCs group compared with the other two groups （P〈0.05）. At the site of high expression of BDNF, the number of apoptosis cells decreased markedly. Conclusion： BDNF gene can promote the differentiation of the stem cells into neurons rather than glial cells, and enhance neuromotor function after brain injury.

关 键 词：Brain-derivedneurotrophicfactor Stem cells Cell differentiation Brain injuries 

分 类 号：R651.1[医药卫生—外科学]