核酶对鸭乙型肝炎病毒感染体内抗病毒作用效果的观察  

The effect of ribozyme RzDS on duck hepatitis B virus

作　　者：李虹[1,2] 陈文[1,2] 徐纬 王平[1,2] 郭巨涛 侯云德 

机构地区：[1]中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室 [2]中国医学科学院医药生物技术研究所

出　　处：《中华实验和临床病毒学杂志》1998年第2期118-121,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家863高科技生物技术领域基金

摘　　要：目的观察核酶在体内抗病毒作用效果。方法选择鸭乙型肝炎（ＤＨＢＶＬＪ－７６）及其鸭动物模型作为检测系统，将针对ＤＨＢＶＰｒｅ－Ｓ７３６位点的核酶（ＲｚＤＳ）插入ｐＪ１２０质粒构建成ｐＪ－ＲｚＤＳ。与痘苗病毒天坛７６１株（ＶＶ）同源重组后获得含ＲｚＤＳ的重组痘苗病毒（Ｖ－ＲｚＤＳ）。用ＤＨＢＶ感染１日龄北京鸭，分别在感染的同时、感染后２４小时和７２小时注射Ｖ－ＲｚＤＳ和ＶＶ，１０天后检测血清中ＤＨＢＶＤＮＡ和ＤＨＢｓＡｇ的含量。结果北京鸭在感染ＤＨＢＶ的同时注射Ｖ－ＲｚＤＳ，其ＤＨＢＶＤＮＡ和ＤＨＢｓＡｇ的含量均低于ＶＶ对照组，两组之间有显著性差异。结论提示ＲｚＤＳ对ＤＨＢＶ基因组复制和表达均有抑制作用，其抑制率分别为４５％和６３％。To study the effect of ribozyme in vivo,the hammerhead ribozyme RzDS was designed and synthesized,which aims at the pregenome RNA of DHBV(LJ-76) at the 736 site of the genome.The RzDS cleaved efficiently its substrate in vitro.We constructed pJ-RzDS,in which RzDS was inserted into pJ120 plasmid under the control of Pll promoter.After homogenous recombination with the vaccinia virus,Tian Tan strain,we got the recombinant(V-RzDS) which carried RzDS.V-RzDS was injected into nestling Beijing ducks preinfected with DHBV. On the tenth day after intravenous injection,DHBV DNA and DHBsAg in serum were detected.The quantity of DHBV DNA and DHBsAg were evidently lower than that of the controls, when V-RzDS injection was simultaneously given with DHBV.It suggests that RzDS can block replication and expression of DHBV in vivo.

关 键 词：核酶 鸭乙型肝炎病毒 复制 表达 

分 类 号：R373.21[医药卫生—病原生物学]