血平板纸片法分离嗜血杆菌的方法学研究  

A novel method for isolating haemophilus species on blood agar

作　　者：唐明忠[1] 刘娟[1] 王美荣[1] 吴静[1] 

机构地区：[1]首都医科大学附属北京天坛医院检验科

出　　处：《中华医学检验杂志》1998年第4期207-209,共3页

摘　　要：目的建立一种简便、有效的分离嗜血杆菌的方法。方法利用万古霉素杆菌肽氯林可霉素巧克力法（ＶＢＣ法）、血平板纸片法和传统巧克力法，对含正常菌群的标本进行嗜血杆菌的分离。结果巧克力平板上流感嗜血杆菌生长佳，但正常菌群影响嗜血杆菌分离，对１００份模拟痰标本分离情况进行比较，结果ＶＢＣ法、纸片法及巧克力法的分离率分别为７６％、８４％和６４％，进行配对卡方检验，巧克力法与ＶＢＣ法和纸片法的Ｐ值均小于０．０１，其结果差异有显著性；对２４１份临床痰标本进行ＶＢＣ法和纸片法分离，流感嗜血杆菌分离率分别为４．３％和４．９８％，进行卡方检验，Ｐ＞０．０５，两法结果无差异。结论血平板上纸片分离嗜血杆菌的方法简便、实用、效果较好，初次分离时可观察卫星现象和溶血情况。Objective To establish a simple and effective method for isolation of Haemophilus species. Methods Haemophilus species from specimens contaminated by microbial flora were isolated with a strip method on blood agar, a conventional chocolate agar method, and a chocolate agar with vancomycin, bacitracin and clidamycin(CHOCVBC) method.Results Haemophilus influenzae grew well on the conventional chocolate agar. But microbial flora had influence on the growth of Haemophilus species. According to the result of 100 simulative sputum specimens isolation, the recovery rates of H.influenzae with CHOCVBC, the strip method on blood agar, and chocolate agar were 76%,84%, and 64% respectively. The recovery rate of H. influenzae with chocolate agar was significantly lower than that with the strip method and CHOCVBC (P<0.01). H. influenzae were isolated from 241 clinical sputum specimens by CHOCVBC and the strip method. The rates of recovery were 4.3% and 4.98% respectively. The two methods showed no significant difference (P>0.05). Conclusion The strip method to isolate Haemophilus spp. on blood agar is effective and simple. The satellite phenomenon and hemolysis can be observed during the primary isolation.

关 键 词：嗜血杆菌属 培养基 血平板纸片法 

分 类 号：R378.4[医药卫生—病原生物学] R446.5[医药卫生—基础医学]