山东省原发性高血压2号染色体基因扫描研究  

A Search for Susceptibility Loci to Essential Hypertension on Chromosome 2 in Hypertensive Patients in Shandong Province

作　　者：周鹏[1] 陈刚[1] 王博[1] 张成[2] 刘文敏[1] 魏然[1] 栾萌[1] 杨树林[1] 刘阳[1] 高春义[1] 

机构地区：[1]山东省医学科学院基础医学研究所医学遗传学重点实验室,山东济南250062 [2]山东省济南市第五人民医院,山东济南250062

出　　处：《中华高血压杂志》2009年第7期641-644,共4页Chinese Journal of Hypertension

基　　金：山东省医学科学院基金项目0617

摘　　要：目的对山东省原发性高血压(EH)患者群体的2号染色体进行扫描,寻找关联区域,定位易感基因。方法用DNA混合池(DNApooling)方法,在2号染色体上间隔10cM(厘摩)遗传距离选择30个微卫星遗传标记,对450例EH患者和450例正常对照者组成的DNA混合样本分别进行扫描。采用Clump软件进行统计学分析,比较患者组与对照组每个等位基因频率的差异。结果在D2S2211(2p25.1)位点患者组与对照组的等位基因频率差异有统计学意义(P<0.01)。结论山东省EH患者群体在2号染色体D2S2211位点存在关联,附近可能存在易感基因,需进一步筛查。Objective A search for susceptibility loci to essential hypertension on chromosome 2 was performed for finding susceptibility loci for essential hypertension. Methods A total of 30 microsatellite markers on chromosome 2 spaced at about 10 cM were selected and two separated DNA pooling samples consisting of 450 essential hypertension eases and 450 normal controls were genotyped respectively. Statistic analysis was performed by Clump software to compare the difference in allele frequency between two pooled samples. Results Significant difference in alleles frequency was found at D2S2211 between hypertensives and controls with P〈0.01. Conclusion D2S2211 on chromosome 2 is associated with essential hypertension in hypertensive patients in Shandong province and candidate genes need to be checked around this locus.

关 键 词：原发性高血压 2号染色体 基因 DNA混合池 

分 类 号：R544.1[医药卫生—心血管疾病]