慢性丙型肝炎患者血清和肝组织中庚型肝炎病毒RNA检测  

PCR detection of hepatitis G virus RNA in sera and liver tissues from patients with chronic hepatitis C

作　　者：于建国[1,2] 侯宪荣[1,2] 潘伟 张光曙 周秀梅[1,2] 

机构地区：[1]解放军88医院传染科 [2]第二军医大学微生物教研室

出　　处：《华人消化杂志》1998年第7期580-581,共2页

摘　　要：目的了解慢性丙型肝炎（ＣＨＣ）患者血清和肝组织中庚型肝炎病毒（ＨＧＶ）感染的状况．方法用ＲＴＰＣＲ方法对４９例ＣＨＣ患者血清和其中１４例肝组织进行ＨＧＶＲＮＡ检测．结果在４９例ＣＨＣ患者的血清和其中１４例肝组织中ＨＧＶＲＮＡ阳性检出率分别为１２２％和２１４％，证实ＨＧＶ和ＨＣＶ感染在ＣＨＣ患者血清和肝组织中同时存在，而肝组织中ＨＧＶＲＮＡ阳性检出率更高，其原因同受血或其直接经血液暴露密切相关．结论ＣＨＣ患者血清和肝组织中存在ＨＧＶ感染，而且感染率较高，故应加强对献血员进行ＨＧＶＲＮＡ和ＡＬＴ筛查。AIM To find out the condition of HGV infection in sera and liver tissues from patients with chronic hepatitis C. METHODS RT PCR was used to detect the HGVRNA in the sera from 49 patients and liver tissues from 14 patients with chronic hepatitis C. RESULTS In the sera of 49 patients and in the liver tissues of 14 patients with chronic hepatitis C, the positive detectable rates of HGVRNA were 12 2% (6/49) and 21 4% (3/14) respectively, which showed that HGV exists simultaneously in the sera and liver tissues of patients with chronic hepatitis C. The reason of the higher positive detectable rate of HGVRNA in the liver tissues was that HGV and HCV infection was closely related to transfusion and other direct exposure to blood. CONCLUSION There are HGV and HCV infections in patients with chronic hepatitis C in southern Shandong, with a high detectable rate. It is imperative to further screen blood donors for HGVRNA and HCVRNA (PCR) and ALT so as to control HGV and HCV infection via blood transfusion.

关 键 词：丙型肝炎 病毒学 肝炎病毒 遗传学 分离 提纯 

分 类 号：R512.63[医药卫生—内科学]