套式PCR技术用于疟疾诊断的研究  

NESTED PCR APPLIED FOR DETECTING MALARIA

作　　者：许国君[1] 诸欣平[2] 董洁[2] 周蕾 李明[3] 康杨[1] 

机构地区：[1]四川省寄生虫病防治研究所,成都610041 [2]首都医科大学寄生虫学教研室,北京100054 [3]第一军医大学热带医学研究所,广州510515

出　　处：《实用寄生虫病杂志》1998年第2期55-57,共3页Journal of Practical Parasitic Diseases

摘　　要：采用三对引物建立了套式PCR疟疾诊断技术，对来源于同一地区的34例门诊镜检确诊为间日疟的血标本及国外输入的2例恶性疟的血标本进行了病原体检测和虫种鉴定，得到预期扩增的DNA片段，经琼脂糖凝胶电泳，显示出肉眼可见的与引物对应的扩增区带，与镜检结果一致。Nested polymerase chain reaction has been applied for detecting rnalaria by using 3 primers synthesised with reference to the SSU rRNA gene sequence of human Plasmdium. Blood samples from 34 proved vivaxmalaria cases in Mingshan County, Sichuan and 2 imported falciparum malaria cases were collected for Plasmodum detection and species identification. The results showed that a 120 bp amplified DNA fragment of P.vwax and 205 bp of P. falciparum were obtained and relevant bands could be seen by naked eye in argarosegel electrophoresis.

关 键 词：疟疾 DNA PCR 诊断 

分 类 号：R531.304[医药卫生—内科学]