灵芝对糖尿病大鼠肾组织氧化应激的影响  

Effect of Ganoderma lucidum on the Oxidative Stress in the Kidney in Diabetic Rat Kidney

作　　者：毛春谱[1] 李小毅[1] 张红梅[1] 林桂芬[1] 李伟[2] 

机构地区：[1]苏州大学附属无锡市第四人民医院内分泌科,江苏无锡214062 [2]徐州医学院附属医院内分泌科,江苏徐州221002

出　　处：《四川中医》2009年第7期27-29,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：江苏省中医药局资助项目(编号:H05102)

摘　　要：目的:观察灵芝对糖尿病大鼠肾组织氧化应激的影响。方法:30只雄性SD大鼠随机分为3组:正常对照组(C组),糖尿病未治疗组(DM组),糖尿病灵芝(1.2g.kg-1)治疗组(GL组)。腹腔注射链脲佐菌素(STZ)诱导大鼠造成糖尿病模型,GL组用灵芝进行治疗性灌胃。8周后,所有大鼠一次性处死。检测肾组织丙二醛(MDA)的含量、肾组织超氧化物歧化酶(SOD)及过氧化氢酶(CAT)与谷胱甘肽过氧化物酶(GSH-Px)活性;同时进行光镜、透射电镜观察各组大鼠肾皮质病理改变。结果:DM组大鼠出现糖尿病肾病(DN)典型的病理改变。GL组肾组织MDA含量明显低于DM组,SOD、CAT、GSH-Px活性明显高于DM组(P<0.01),病理改变好转。结论:灵芝可能通过抑制肾组织氧化应激,起到对糖尿病大鼠肾脏的保护作用。Objcective： To investigate the effect of Ganederma lucidum （GL） on the oxidative stress in the kidney in diabetic rots. Methods： Thirty Sprague - Damly rats were randomly divided into three groups： normal control group （ C group ）, diabetes mellitus group （DM group） and diabetic group treated with GL （1.2g·kg^-1）（GL group） . Diabetic model was induce by streptezocin （60mg·kg^-1 ） and remedial peffusing stomach by administrating GL was done on the GL group. Rats were killed at 8 weeks and kidneys were taken out.The activities of antioxidant enzymes including maleie dialdehyde （MDA）, superoxide dismutsse （SOD ）, catalase （CAT） and glutathione peroxidase （GSH- Px） in kidney tissure was determined.Renal cortex tissue morphology were obwerved by light microscope and transmission electron microscope.Results： Levels of MDA were higher and SOD, CAT as well as GSH-px activities wree significantly lower in glomeruli in DM group rats than those in C group. GL administration could all remit these changes （ P 〈 0.01） .Meanwhile, the pathologic changes of glomeruli were serious in DM greup.The abnormal expressions of these proteins and pathologic changes were dramafically improved in diabetic rats by GL treatment. Conclusion： GL could exert protective effects on diabetic nephropathy, partly through correlation with the suppression of increase in oxidative stress in diabetic rats.

关 键 词：灵芝 糖尿病肾病 氧化应激 

分 类 号：R285.6[医药卫生—中药学]