RGD肽表面修饰壳聚糖载基因纳米粒子的体外实验研究  

In vitro study on RGD-immobilized chitosan nanoparticles as gene vectors

作　　者：唐锋[1] 管珩[1] 刘昌伟[1] 李拥军[1] 郑月宏[1] 李晓慧[2] 鲍军波[2] 唐丽娜[2] 宋存先[2] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院血管外科,北京100032 [2]中国医学科学院北京协和医学院生物医学工程研究所,天津300192

出　　处：《国际生物医学工程杂志》2009年第3期129-132,I0001,共5页International Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目（30772114.50830106）

摘　　要：目的将Arg—Gly—Asp（RGD）肽偶联到壳聚糖（CH）材料表面，并制备成包载质粒DNA的纳米粒子，以未偶连RGD的壳聚糖载质粒DNA作为对照，进行体外内皮细胞转染，观察其是否能提高对内皮细胞的转染效率。方法以1-乙基-3-（3-二甲基氨基丙基）碳化二亚胺盐酸盐（EDC）和N-羟基丁二酰亚胺（NHS）为偶联剂，通过酰胺键将RGD肽偶联到壳聚糖表面，对其进行表征，并以未偶连RGD的壳聚糖作为对照，制备载pEGFP-C1质粒DNA纳米粒子，比较2者对Hy926细胞的转染效率。结果壳聚糖-RGD（CH-RGD）载基因纳米粒子转染Hy926细胞的效率明显高于未偶连RGD的壳聚糖载基因纳米粒子（35．7％VS14．3％．P〈0．001）。结论RGD肽表面修饰壳聚糖载基因纳米粒子可用于体外细胞转染，其对细胞的转染效率明显优于未偶连RGD的壳聚糖。Objective To investigate gene transfection efficiency in cell culture mediated by chitosan nanoparticles immobilized with Arg-Gly-Asp（arginine-glycine-aspartic acid,RGD）-peptides. Mothods RGD- peptides was immobilized on chitosan by chemical crosslinking. Plasmid pEGFP-C1 was encapsulated into Chitosan-RGD-nanoparticles （Chitosan-RGD- pEGFP-NPs） by complex coacervation method. Chitosan-pEGFP- nanoparticles without RGD were used as the control to evaluate gene tranfection efficiency into Hy926 cells in vitro. Results The transfection efficiency of Chitosan-RGD- pEGFP-NPs is statistically higher than ChitosanpEGFP-NPs.（ 35.7% vs 14.3%,P〈0.001） Conclusion Chitosan-RGD NPs could act as gene vector to transfect specific gene in vitro and the transfection efficiency is higher than that of non-RGD chitosan nanoparticles.

关 键 词：RGD肽 壳聚糖 纳米粒子 基因载体 

分 类 号：R318.08[医药卫生—生物医学工程]