原位胶原凝胶对大鼠脊髓损伤后功能恢复的影响  

Effect of in situ gelling collagen on restoration of function after spinal cord injury in rats

作　　者：李长文[1] 郑启新[1] 吴永超[1] 郭晓东[1] 李景峰[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022

出　　处：《国际生物医学工程杂志》2009年第3期133-136,152,I0001,共6页International Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目（30500511）

摘　　要：目的研究脊髓损伤后原位形成胶原凝胶对神经功能恢复的影响。方法15只健康雌性SD大鼠随机分为胶原组、对照组和假手术组．Allen撞击法制作脊髓损伤模型。用微量注射器将胶原溶液注入损伤部位，在体温作用下让其原位形成凝胶，对照组注入PBS溶液，每周进行运动评分，6周后取脊髓组织进行免疫荧光染色，观察损伤部位的胶质瘢痕和轴突生长情况。结果胶原组大鼠第5周起BBB评分显著高于对照组，免疫荧光显示损伤部位胶质瘢痕少于对照组，且长入损伤部位的轴突也多于对照组。结论原位形成的胶原凝胶能抑制损伤部位胶质瘢痕的形成，并能促进轴突再生长入损伤部位。证明胶原是一种较好的能用于修复脊髓损伤的可注射材料。Objective To explore the effects of in situ gelling collagen on restoration of neuron function after spinal cord injury, in rats. Methods Fifteen healthy female SD rats were randomly divided into collagen group, control group and sham operation group. Contusive spinal cord injury models were made by improved Allen＇s weight drop method. In collagen group and control group, collagen and PBS solutions were respectively injected into epicenter using micro-syringes, and allowed for gel formation in situ at body temperature. BBB locomotion scores were tested once per week for 6 weeks. After 6 weeks, immunohistochemistry was carried out to assess glial scar formation and axon regeneration. Results BBB scores of collagen group were significantly higher than that of control group from 5th week. Fewer glial scars were found in injured area in collagen group and more axons regenerated into injured area than that of control group. Conclusion In situ gelling collagen can inhibit the formation of glial scars in injured area and promote axon regeneration into injured area. Collagen might be a promising injectable material for the repairing of spinal cord injury.

关 键 词：胶原 脊髓损伤 轴突再生 胶质瘢痕 

分 类 号：R651.2[医药卫生—外科学]