漏声表面波生物传感器检测系统构建时靶序列浓度的影响  

Effects of Target Concentrations on Leaky Surface Acoustic Wave Biosensor Detection System

作　　者：陈鸣[1] 李书君 府伟灵[3] 陈伟[1] 张烨[3] 王云霞[3] 徐清华[1] 丁毅[4] 曹亮[4] 

机构地区：[1]第三军医大学第三附属医院检验科,重庆400042 [2]邢台人民医院检验科,河北邢台054000 [3]第三军医大学第一附属医院检验科,重庆400038 [4]中国电子科技集团公司第二十六研究所第五研究室,重庆400060

出　　处：《中华医院感染学杂志》2009年第14期1809-1811,共3页Chinese Journal of Nosocomiology

基　　金：国家863计划重大专项(2007AA02Z416);国家自然科学基金(30400107);军队"十一五"课题(06G073)

摘　　要：目的探讨新型的漏声表面波(LSAW)传感器检测系统构建时,人乳头状瘤病毒(HPV)靶序列浓度对其杂交效应和反应时间的影响。方法LSAW传感器表面先固定上1.0μmol/L的肽核酸(PNA)探针,观察终浓度为1pg/L～1mg/L的HPV靶序列DNA与探针进行杂交所引起的相位变化及反应所需时间,并对相位下降值与靶序列浓度做线性回归,确定其线性范围。结果随着靶序列浓度从1pg/L增加到1mg/L,杂交反应引起的相位下降值呈先增加后趋于缓和的趋势,以100μg/L为分界线,而杂交平衡时间并没有显现出一定的变化趋势;在1pg/L～100μg/L的HPV靶序列浓度范围内,相位变化与靶序列浓度的线性回归方程为△P=2.3392lgC+14.584,相关系数r2=0.9622。结论随着靶序列浓度的升高,杂交反应引起的相位下降值呈典型饱和曲线趋势,靶序列浓度的线性检测范围为:1pg/L～100μg/L。OBJECTIVE To investigate the hybridization efficiency and equilibrium time of human papilloma virus （HPV） target concentrations on the new style leaky surface acoustic wave （LSAW） sensor detection system. METHODS A concentration 1.0 μmol/L peptide nucleic acid （PNA） probe was firstly immobilized on the surface of LSAW sensor. Then target HPV genomic DNA varied from 1pg/L to 1mg/L was hybridized with the probe, and the decrease in phase and equilibrium time of reaction were recorded and analyzed. Linear regression was done between phase decrease and the target concentration; hence the linear range was determined. RESULTS Decrease in the phase enhanced firstly and then tended gently when target concentration varied from 1pg/L to 1mg/L, and 100μg/L was the watershed. And no evident change tendency was observed on equilibrium time for hybridization. The statistic linear regression equation was △P= 2. 3392 lgC＋ 14. 584 among the range of target concentration from 1pg/L to 100μg/L and the correlating coefficient was 0. 9622. CONCLUSIONS The phase change caused by hybridization reaction is a typical saturation curve in accordance with the increase in the target concentration. And the linear range of the target concentration is between 1pg/L to 100μg/L.

关 键 词：肽核酸 探针 漏声表面波 传感器 杂交 

分 类 号：R446.5[医药卫生—诊断学]