猪繁殖与呼吸综合征病毒nsp2基因缺失株RT-PCR检测方法的建立  

Establishment of RT-PCR for detection of nsp2 deletion porcine reproductive and respiratory syndrome virus

作　　者：李玉峰[1] 王先炜[1] 陈闻[1] 姜平[1] 许家荣[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《南京农业大学学报》2009年第3期169-171,共3页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30871868);南京农业大学青年科技创新基金(KJ07014)

摘　　要：为了快速检测猪繁殖与呼吸综合征病毒(PRRSV)nsp2基因缺失流行株,在对不同PRRSV分离株nsp2基因核苷酸序列分析的基础上,设计了针对nsp2基因的1对兼并PCR引物和1条基因特异性反转录引物。RT-PCR试验结果表明,经典PRRSVS1毒株和高致病性nsp2缺失株SY0608毒株的PCR扩增片段的大小分别为768 bp和678 bp。特异性试验和敏感性试验证明,该检测方法特异性较好,但敏感性相对较低,可用于快速鉴别诊断PRRSVnsp2基因缺失毒株和经典PRRSV毒株。In order to rapidly detect nsp2 gene deleted isolates, based on nsp2 gene sequences of different isolates accessed in Gen- Bank, a pair of degenerate PCR primers and one gene specific reverse primer were designed. RT-PCR results showed that the PCR products for nsp2 deleted PRRSV and conventional isolates were 768 bp and 678 bp in length, respectively. Sensitivity and speci- ficity tests results showed this method had good sensitivity and specificity. It could be used to rapidly differentiate nsp2 deleted iso- lates and conventional PRRSV isolates.

关 键 词：PRRSV NSP2基因 RT—PCR 基因缺失 

分 类 号：S852.65[农业科学—基础兽医学]