杜仲内生真菌的分离鉴定及其抑菌活性研究  

Isolation and Identification of Endophytic Fungi from Eucommia ulmoides and Study of Their Antimicrobial Activities

作　　者：王丽丽[1] 白方文[1] 张西玉[2] 陈晓敏[1] 董桂灵[1] 白林含[1] 

机构地区：[1]四川大学生命科学学院,四川成都610065 [2]乐山师范学院化学与生命科学系,四川乐山614000

出　　处：《四川师范大学学报（自然科学版）》2009年第4期508-512,共5页Journal of Sichuan Normal University（Natural Science）

基　　金：国家科技部项目(2005DKA21208-7)资助项目

摘　　要：从药用植物杜仲中分离到41株内生真菌,综合运用形态特征和分子生物学技术,明确其分类地位,分别属于粗糙脉孢霉(Neurospora crassa)、曲霉属(Aspergillus)、小菌核属(Sclerotium)、盾壳霉属(Conio-thyrium)、离蠕孢属(Bipolaris)、白僵菌属(Beauveria)6个属,并进行抗植物病原真菌活性检测,结果表明48.8%的内生真菌对植物病原菌有生长抑制作用,除1株白僵菌属菌株外对G+、G-细菌均有明显的抑菌作用.Forty-one strains of endophytie fungi were isolated from the branches of Eucommia ulmoides in Sichuan province. They were classified and identified to six genera according to their morphological and micro-structure characters, e.g. Neurospora crassa, As- pergillus, Sclerotium, Coniothyrium, Bipolaris, Beauveria. Antibacterial activities of 41 strains endophytic fungi were tested with phyto- pathogen fungi. The result shows that the percentage of antifungal strains is 48.8%. Most strains prominently have inhibitive actions to all tested phytophogen fungi and bacterium（ G ＋ , G- ） except Beauveria.

关 键 词：杜仲 内生真菌 分类鉴定 抑菌作用 

分 类 号：Q93[生物学—微生物学]