SD大鼠胸主动脉平滑肌细胞原代培养及鉴定  

PRIMARY CULTURE AND IDENTIFICATION OF SD RAT AORTIC SMOOTH MUSCLE CELLS

作　　者：陈雄林[1,2] 杨耀防[3] 熊建军[2] 龚帧[2] 李雪芹[2] 石小玉[1] 

机构地区：[1]南昌大学医学院 [2]九江学院基础医学院,江西九江332000 [3]九江学院,江西九江332000

出　　处：《九江医学》2009年第2期1-2,17,共3页Jiujiang Medical Journal

摘　　要：目的:研究SD大鼠胸主动脉平滑肌细胞(RASMC)的培养方法及其生物学特性。方法:运用组织块贴壁法进行SD大鼠胸主动脉平滑肌细胞培养,并用倒置相差显微镜观察、HE染色后形态学观察以及用免疫组化对培养细胞进行鉴定。结果:组织块贴壁法成功培养出SD大鼠胸主动脉平滑肌细胞,培养的细胞呈典型"峰-谷"样生长,HE染色细胞呈梭形,胞浆丰富,核大而圆或椭圆,免疫组化S-P法检测a-平滑肌肌动蛋白单克隆抗体(a-SMActin)呈强阳性表达。传代纯化,细胞生长特性未见异常改变。结论:组织块贴壁法培养的SD大鼠胸主动脉平滑肌细胞生长稳定,培养和纯化可同步进行,简单,方便。OBJECTIVE： To study method of primary culture of SD rat aortic smooth muscle cells （RASMC） and identify their biological properties. METHOD ： SD rat aortic tissue was planted with the adherent method of tissue explants. The cellular morphology was observed by inverted phase contrast microscope and the nucleus and cytoplasm were observed after haematoxylin and eoain （HE） staining. The immunocytoehemistry was used to identify the RASMC. RESULTS： The RASMC were successfully cultured by the adherent method of tissue explants and grown in the ＂peak - valley＂ mode. The cultured RASMC were fusiform shape with a big oviform or round nuclei rich in cytoplasm. lmmunohistochemieal method （ S - P method） showed strong expression of a - smooth muscle actin （ a - SMAetin）. After passage and purification, no abnormality was seen. CONCLUSION： The primary cultured SD rat aortic Smooth Muscle Cells with the adherent method of tissue explants can stably grow. In addition, the culture and sublimation could be in synchronism. The method is simple and convenient.

关 键 词：SD大鼠 胸主动脉平滑肌 原代培养 鉴定 

分 类 号：Q813.11[生物学—生物工程]