丹参酮ⅡA和丹酚酸B抗心脑缺血的比较研究  

Tanshinone ⅡA vs.Salvianolic Acid B Against Cardio-cerebral Ischemia

作　　者：黄凯文[1] 祝维峰[2] 苏宁[3] 郭洁文[2] 邓志军[3] 吴伟文[1] 郝大为[1] 陆晓晖[1] 

机构地区：[1]广东省佛山市顺德区第一人民医院,佛山市528300 [2]广东省广州市中医医院,广州市510130 [3]广州中医药大学,广州市510405

出　　处：《中国医院用药评价与分析》2009年第6期449-452,共4页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：广东省社会发展领域科技计划项目(No.2008B030301266);广东省中医药管理局基金资助项目(No.2007210);广州市中医药中西医结合科研(No.2007A07)联合资助

摘　　要：目的:比较研究等剂量的丹参酮ⅡA、丹酚酸B及其等质量混合物对4种常用心脑缺血整体动物模型的影响,为临床用药选择提供依据。方法:复制垂体后叶素和结扎冠脉所致大鼠实验性心肌缺血模型,双侧颈总动脉结扎所致大鼠实验性脑缺血模型,线栓所致大鼠实验性脑缺血-再灌注模型,按50 mg.kg-1.wt-1灌胃给予丹参酮ⅡA、丹酚酸B及其等质量混合物,观察相应缺血指标。双侧颈总动脉结扎模型尚测定脑组织中相关生化指标,包括一氧化氮(NO)、一氧化氮合酶(NOS)、钠-钾ATP酶(Na+-K+ATPase)、钙-镁ATP酶(Ca2+-Mg2+ATPase)、丙二醛(MDA)。结果:丹参酮ⅡA及混合物对急性心肌缺血的作用优于丹酚酸B,尤其是对垂体后叶素所致的一过性心肌缺血;丹酚酸B仅在缺血时间较长的冠脉结扎模型中表现一定作用。在两种脑缺血模型中,丹酚酸B及混合物均表现较好的拮抗作用,该作用与丹酚酸B强抗氧化能力和对Ca2+-Mg2+ATPase降低的抑制有关。结论:丹参酮ⅡA、丹酚酸B在心脑缺血的治疗中具有不同的作用特点,在相关药物应用时,应根据临床需要进行恰当选择;二者对心、脑缺血均有一定的协同作用,提示其联合用药可提高疗效,提升药物的临床价值。OBJECTIVE： To evaluate the effects of tanshinone Ⅱ A （Tan Ⅱ A）, salvianolic acid （Sal B） and their respective mixture （at equal mass） on four cardio - cerebral ischemia animal models so as to provide basis for clinical use of Tan Ⅱ A and Sal B. METHODS： Pituitrin or coronary ligation induced rat cardio - ischemia models, bilateral common carotid artery occlusion （BCAO） rat cerebral ischemia model and middle cerebral artery occlusion/reperfusion（MCAO） rat cerebral ischemia model were established. The model rats were administered with one of the following drugs： tanshinone A （Tan ⅡA）, salvianolic acid （Sal B） and the mixture of tanshinone Ⅱ A or the mixture of salvianolic acid at a dosage of 50 mg·kg^-1·wt^-1, then the ischemia associated indexes were recorded, and biochemical indexes in brain for bilateral common carotid artery occlusion （BCAO） rat cerebral ischemia model including NO, NOS, Na^＋ - K^＋ ATPase, Ca^2＋ - Mg^2＋ ATPase and MDA were also determined. RESULTS ： The effects of Tan I1 A and its mixture on cute cardio ischemia was better than that of Sal B, especially on pituitrin induced transient myocardial ischemia ; while Sal B showed certain efficacy for prolonged ischemia of coronary ligation model. Sal B and its mixture showed satisfactory anti - ischemic effects in both of the two cerebral ischemia models, which could be associated with its anti - oxidation effect and inhibitory effect on the reduction of Ca^2＋ - Mg^2＋ ATPase. CONCLUSION： Tan Ⅱ A is different from Sal B in action against cardio - cerebral ischemia;therefore, their use should be properly based on clinical needs. The synergistic effect of the two drugs on cardio - cerebral ischemia suggests that the combination of them is conducive to the enhancement of therapeutic efficacy and drugs＇ clinical value.

关 键 词：丹酚酸 丹参酮 缺血 

分 类 号：R972[医药卫生—药品]