α防御素在慢性乙型肝炎患者和HBV携带者之间的差异表达  

Differential expressions of α-defensin between chronic hepatitis B and asymptomatic HBV carriers

作　　者：唐翠兰[1] 陈智[1] 

机构地区：[1]浙江大学医学院附属第一医院传染病诊治国家重点实验室,杭州310005

出　　处：《中华临床感染病杂志》2009年第2期86-88,共3页Chinese Journal of Clinical Infectious Diseases

基　　金：浙江省科技厅重大项目（2003C13015）

摘　　要：目的测定慢性乙型肝炎（CHB）患者和HBV携带者体内差异表达的基因，以揭示二者不同的发病机制。方法采用抑制消减杂交（SSH）技术构建差异表达基因cDNA文库并筛选出差异表达基因α防御素。采集46例CHB和11例HBV携带者外周肝素抗凝血，用实时定量RT—PCR和酶联免疫吸附试验（ELISA）检测α防御素在2组患者中mRNA和蛋白水平的差异表达。结果实时定量RT—PCR显示，α防御素在CHB患者中的表达是在HBV携带者中的1．4倍。ELISA结果显示，血浆α防御素在CHB患者中表达的水平为（216．40±81．25）μg/L，HBV携带者为（156．00±57．26）μg/L，二者比较差异有统计学意义（t=2．23，P〈0．05）。结论α防御素在CHB患者中的表达较HBV携带者高，提示其可能参与了CHB的发病机制。Objective To explore different pathogenesis of chronic hepatitis B （CHB） and asymptomatic HBV carriers （ASCs） by identifying differentially expressed genes. Methods Subtracted library was constructed by suppression subtractive hybridization （SSH）, and α-defensin was identified by dot blot hybridization. Peripheral blood was collected from 46 CHB patients and 11 ASCs, and the expressions of α-defensin mRNA in peripheral blood mononuclear cell and protein in plasma were determined by the real time RT-PCR and enzyme-linked immunosorbent assay （ELISA）. Results Real time RT-PCR showed that the expression of α-defensin mRNA in blood samples of CHB was 1.4-fold higher than that of ASCs. As shown by ELISA, the plasma level of α-defensin in CHB was higher than that of ASCs [ （216. 40 ± 81.25 ） μg/L vs. （ 156. 00 ± 57. 26 ） μg/L, t = 2. 23, P 〈 0. 05 ]. Conclusion α-defensin may involve in the pathogenesis of CHB, for it is over-expressed in CHB patients. [

关 键 词：肝炎病毒 乙型 肝炎 乙型 慢性 无症状HBV携带者 α防御素 

分 类 号：R51[医药卫生—内科学]