培养大鼠脑皮层神经元损伤后c-fos蛋白表达  

Expression of c-fos Protein After Cultured Cortical Neuron Injury in Rats

作　　者：王克万[1,2] 杨志焕 王正国[1,2] 朱佩芳 包新民[1,2] 舒斯云 陈长才[1,2] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所 [2]第一军医大学附属珠江医院神经医学中心

出　　处：《中华创伤杂志》1998年第4期219-222,共4页Chinese Journal of Trauma

摘　　要：目的观察培养的大鼠皮层神经元损伤后ｃ－ｆｏｓ蛋白表达，探讨其在继发性脑损伤中的作用。方法混合培养１０～１２天ＳＤ胎鼠的皮层神经元分别用谷氨酸和机械划痕损伤。采用ＳＰ法进行免疫组化双重标记反应。结果谷氨酸作用后２小时，ｃ－ｆｏｓ蛋白开始表达，４小时后在核中明显浓聚。创伤后２小时仅在创道边缘直接损伤部位有ｃ－ｆｏｓ蛋白表达，远离创道未直接损伤区的神经元在创伤后４小时才出现；少数神经元突起远端有明显ｃ－ｆｏｓ蛋白颗粒；损伤后６小时神经元突起开始崩解，但ｃ－ｆｏｓ蛋白颗粒仍未消失。结论谷氨酸损伤可引起培养的神经元ｃ－ｆｏｓ蛋白的大量表达，损伤后培养皮层神经元和胶质细胞可以释放出神经毒性物质。Aim To observe the expression of c-fos protein in cultured cortical neurons subject to either glutamate or mechanical injury in rats, and to study the effect of c-fos protein on brain injury. Methods Mixed cultured cortical neurons from SD rats were injured by either glutamate or mechanical force, then the expression of c-fos protein was studied with doublelabeled immunohistochemistry. Results Two hours after glutamate injury, the expression of c-fos protein began in the nuclei and 4 hours later it reached peak, and c-fos protein only expressed in the direct injured area at the edge of traumatic tracts after 2 hours and began to express till 4 hours in the indirect injured area far from traumatic tracts. The positive immunoreaction of c-fos protein granule remained. Conclusion The c-fos protein expression can be induced by glutamate. Neurotoxic substances were produced in cultured medium after traumatic injury, and c-fos expression may be associated with neuron apoptosis.

关 键 词：C-FOS蛋白 神经元培养 谷氨酸 继发性 脑损伤 

分 类 号：R651.15[医药卫生—外科学]