促甲状腺素释放激素治疗实验性脊髓损伤的酶及免疫组织化学评价  

Evaluation of Enzyme Histochemistry and Immunohistochemistry on TRH in Treating Experimental Spinal Cord Injury

作　　者：朱悦[1,2] 王星铎[1,2] 包峰[1,2] 

机构地区：[1]中国医科大学第一临床学院骨科 [2]中国医科大学脑研究所神经解剖研究室

出　　处：《中华创伤杂志》1998年第4期230-232,共3页Chinese Journal of Trauma

基　　金：辽宁省科学技术委员会基金

摘　　要：目的为促甲状腺素释放激素（ＴＲＨ）对实验性脊髓损伤的治疗作用提供可靠的形态学依据。方法以改良Ａｌｅｎ法制作Ｗｉｓｔａｒ大鼠不完全性脊髓损伤模型；采用定量酶组织化学方法比较正常组、治疗组和对照组脊髓前角细胞内乙酰胆碱酯酶（ＡｃｈＥ）、酸性磷酸酶（ＡＣＰ）活性；应用定量免疫组织化学方法比较脊髓白质内轴突数量；通过超微结构观察比较脊髓组织损伤程度。结果（１）损伤后不同时间治疗组前角细胞ＡｃｈＥ活性均高于对照组，损伤后２周基本恢复正常；而ＡＣＰ活性均低于对照组，于损伤后４周基本恢复正常。（２）治疗组脊髓白质一定面积内轴突数量多于对照组。（３）超微结构观察显示治疗组神经组织损伤及出血程度均轻于对照组。Aim To provide reliable morphological evidence for therapeutic effects of thyrotropinreleasing hormone (TRH) on the experimental spinal cord injury. Methods Wistar rats were subjected to incomplete spinal cord injury with the modified Allen methods; Acetylcholinesterase (AchE) activity and acid phosphatase (ACP) activity of anterior horn cells in the normal group, the treated group and the control group were measured respectively with the quantitative enzyme histochemistry; The number of axon in the spinal white matter was surveyed in the three groups by means of the quantitative immunohistochemistry; The spinal tissue injury was also observed ultrastructurally. Results (1) AchE activity of anterior horn cells in the treated group was higher than that of the control group at various time, and its activity restored to normal level basically two weeks after injury; ACP activity was lower than that of the control group in various time, and its activity restored to normal level basically four weeks after injury. (2) The number of axon in a certain area of spinal white matter in the treated group was more than that of the control group. (3) Ultrastructural observation showed that both the nerve tissue injury and the extent of hemorrhage were milder in the treated group than that of the control group. Conclusion The therapeutic effects of TRH on the experimental spinal cord injury have the reliable morphological evidence.

关 键 词：脊髓损伤 酶组织化学 免疫组织化学 TRH 

分 类 号：R651.205[医药卫生—外科学]