基于包埋在DNA-碳纳米管复合物中的HRP修饰电极的过氧化氢传感器的研制  

A New Biosensor Based on Horseradish Peroxidase Immobilized on SCNTs and DNA Complex Composite Film on a Carbon Electrode

作　　者：严楠[1] 宋永海[1] 汪莉[1] 崔康[1] 

机构地区：[1]江西师范大学化学化工学院,江西南昌330022

出　　处：《江西师范大学学报（自然科学版）》2009年第3期257-260,共4页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(20705001);江西省教育厅科技项目(赣财教[2007]127);江西师范大学科研启动基金;江西师范大学大学生学术课题资助项目

摘　　要：将辣根过氧化物酶(HRP)包埋于DNA-碳纳米管复合物中后再修饰到玻碳电极上制得过氧化氢传感器.DNA和碳纳米管的复合物为酶分子提供了一个生物兼容性很好的微环境,使HRP在电极上的生物活性大大增强.此修饰电极对过氧化氢的还原表现出了很好的催化能力,同时我们还对其它因素影响进行了研究.实验表明,此传感器能在很短的时间(5 s)内对过氧化氢有很好的响应.其检测线为2.0 mol/L,检测范围为10.0μmol/L^10.2 mmol/L.A novel method to fabricate a hydrogen peroxide sensor was developed by immobilizing horseradish peroxidase（HRP） on a carbon nanotubes（ CNTs）-DNA compatible film which were directly dropped on glass carbon electron（GCE）. The DNA-CNTs composite film obtained provided a biocompatible microenvironment for enzyme molecules and a tunnel to transfer electron. The immobilized HRP displayed good electrocatalytic response to the reduction of hydrogen peroxide. The performance and factors influencing the resulted sensor were studied in detail. The resulted biosensor exhibited fast amperometric response （within 5 s） to H2O2. The detection limit of the sensor was 2.0 μmol/L, and linear range was from 10.0 μmol/L to 10.2 mmol/L.

关 键 词：辣根过氧化酶(HRP) 碳纳米管(CNTs) DNA 玻碳电极 GCE.生物传感器 

分 类 号：O657.15[理学—分析化学]