不同理化因子对分离于荒漠生物结皮中念珠藻生长的影响  

Influences of Different Physico-chemical Factors on Nostoc sp. Separated from Biological Soil Crusts

作　　者：郑云普[1] 张丙昌[2,3] 赵建成[1] 张元明[2] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016 [2]中国科学院新疆生态与地理研究所,新疆乌鲁木齐830011 [3]中国科学院研究生院,北京100039

出　　处：《河北师范大学学报（自然科学版）》2009年第4期531-537,542,共8页Journal of Hebei Normal University：Natural Science

基　　金：国家自然科学基金(30670152;40571085);河北省自然科学基金(C2008000158);中国科学院知识创新工程重要方向项目(KZCX3-SW-343);中国科学院研究生科学与社会实践资助项目

摘　　要：通过对分离于生物结皮中的念珠藻(Nostoc sp.)进行室内培养,研究了K+浓度、Ca2+浓度、Mg2+浓度、pH值、温度、光照等理化因子对其生长的影响,初步探讨了念珠藻生长所需的最适生理条件.研究结果表明,K+浓度、Ca2+浓度对念珠藻生长的影响为显著水平,pH值、Mg2+浓度对念珠藻生长的影响为极显著水平,温度和光照强度对念珠藻的影响不显著.多因素方差分析结果进一步表明,pH值、Ca2+浓度、Mg2+浓度对念珠藻生长的影响呈极显著水平,K+浓度对念珠藻生长的影响不显著.念珠藻的最适生长条件为温度25℃,光照强度66μmol.m-2.s-1,pH=11,K+浓度为5.38×10-5mol/L,Ca2+浓度为1.77×10-5mol/L,Mg2+浓度为1.02×10-4mol/L.The influences of K ＋ , Ca2 ＋ , Mg2 ＋ , pH value, temperature,light intensity on the growth of Nostoc sp. were studied by isolating, purifying and culturing under laboratory conditions. Physiological and ecological characteristics of Nostoc sp. were investigated primarily. The results revealed that the growth of Nostoc sp. was significantly influenced by K ＋ and Ca2 ＋ , while Mg2 ＋ was highly significant. There were highly significant difference under different pH and no difference under different temperature and light intensity. The results of multivariate variance analysis showed that pH value, Ca2＋ , Mg2＋ had highly significant effect on the growth of Nostoc sp. ,but K ＋ had no difference. In addition, the results also showed that the most favourable culturing conditions were at temperature 25℃ ,light intensity 66μmol·m-2·s-1 ,pH 11 ,K＋ concentration 5.38×10^-5 mol/L,Ca2＋ concentration 1.77×10^-5 mol/L and Mg2＋ concentration was 1.02 ×10^-4mol/L.

关 键 词：生物结皮 念珠藻 理化因子 生物量 

分 类 号：Q945.3[生物学—植物学]