铁皮石斛试管苗培养技术的研究  

Study on Vitro Culture Techniques of Dendrobium candidum

作　　者：陈媛[1] 谢吉容[1] 

机构地区：[1]重庆文理学院生命科学与技术学院,重庆永川402160

出　　处：《北方园艺》2009年第7期122-124,共3页Northern Horticulture

基　　金：重庆市自然科学基金资助项目(CSTC;2008BB1205);重庆文理学院学生科技研究重点资助项目(XZ2008014)

摘　　要：研究了不同量激素浓度、天然添加物及植入密度对石斛试管苗生长的影响。结果表明：使用1/2MS培养基并且在其他条件不变的情况下,当6-BA的含量在3.0 mg/L时,丛生芽数量多且有原球茎的发生 香蕉汁具有明显的壮苗和诱导芽增殖的效果 当铁皮石斛茎段植入密度为10~12个/瓶时,对其分蘖和生长有利。Dendrobium candidum is one of the rare Chinese herbal medicines which are in danger. In order to further understanding the artificial propagation system, the experiment studied the effects of different hormone levels, nature addictives and implantation densities. The results Showed that in the same circumstance, using 1/2 MS culture medium, when 6-BA in the content of 3.0 mg/L, there was a larger number of buds and also PLBs occur, banana juice had an obvious effect on sprouts strength and bud-induced proliferation, it is good for the tiller and the growth of Dendrobium candidum, when the implantation density was 10-12/bottle.

关 键 词：铁皮石斛 组织培养 激素 香蕉 植入密度 

分 类 号：S567.219[农业科学—中草药栽培]