牦牛黑素皮质素受体-4基因的克隆与序列分析  

Cloning and sequence analysis of melanocortin receptor-4 gene in yak

作　　者：宋海峰[1] 魏雅萍[1] 马志杰[1] 钟金城[2] 罗晓林[3] 陈雪梅[2] 

机构地区：[1]青海大学畜牧兽医科学院,青海西宁810016 [2]西南民族大学生命科学与技术学院,四川成都610041 [3]四川省草原科学研究院,四川成都611731

出　　处：《青海大学学报（自然科学版）》2009年第3期43-50,共8页Journal of Qinghai University(Natural Science)

基　　金：青海省科技厅犏牛产业化项目(2005-N-108)

摘　　要：对牦牛黑素皮质素受体-4(MC4R)基因进行了克隆和序列分析,以期为进一步开展牦牛MC4R基因与其肥胖性状的相关分析以及基因定位、表达调控等研究提供理论基础。首先采用特定引物对牦牛MC4R基因进行PCR扩增、克隆和测序,然后用B ioEd it7.0.0软件拼接MC4R基因全序列,用DNAMAN5.2.2软件对编码序列进行翻译,用DNAStar6.13比对后比较基因和氨基酸序列同源性。实验获得牦牛MC4R基因全长1343bp,其中编码序列全长999bp,共编码332个氨基酸残基的蛋白质。牦牛MC4R基因序列与普通牛、人、食蟹猴、猪、狗、大鼠、小鼠、鸡、斑马鱼、金鱼、东方鲀和河豚的同源性分别是99.5%、85.5%、84.9%、88.1%、86.7%、83.9%、83.8%、75.5%、61.2%、59.5%、65.3%和64.6%,由此推导的氨基酸序列同源性分别是99.1%、91.9%、91.9%、93.1%、92.2%、90.4%、89.8%、84.0%、66.7%、65.7%、67.2%和66.7%。本研究成功的克隆了牦牛的MC4R基因,表明其在物种间具有较高的保守性。Melanocortin Receptor- 4 （MC4R） Gene in Yak was cloned and sequenced, in order to provide theoretical foundation for further research of the corelevantion of MC4R gene in yak and fat traits,location and expression i.e. MC4R gene of yak was amplified using particular primers,cloned by T - A method and sequenced, and spliced by using BioEdit7.0. 0. then translated by using DNAMAN5. 2. 2. All MC4R sequences were aligned and compared in percent identity by using DNAStar6. 13. The length of MC4R gene in yak was 1343bp, in which the length of complete coding sequence （CDS） was 999bp, encoding 332 amino acids. Homology of nucleotide sequence between yak and cattle, human, crab - eating macaque,pig, dog,rat,mouse,chicken,zebrafish,goldfish,fugu rubripes and tetra is 99.5% ,85.5% ,84.9%, 88. 1% ,86. 7% ,83.9% ,83.8% ,75.5% ,61.2% ,59.5% ,65.3% and 64. 6% respectively.Homology of amino acid sequence of them is 99. 1% ,91.9% ,91.9% ,93. 1% ,92. 2% ,90. 4%, 89. 8% ,84.0% ,66.7% ,65.7% ,67.2% and 66. 7% respecxvely. MC4R gene of yak was successfully cloned in this study, indicating it was quite conservative among species.

关 键 词：牦牛 MC4R基因 序列分析 同源性 

分 类 号：S823.85[农业科学—畜牧学]