显齿蛇葡萄叶总黄酮对苯并芘染毒小鼠血清和肝脏抗氧化酶的影响  

Study on the Effect of Flavonoids of Ampelopsis Grmsedntara Leaves on the Serum and Liver Antioxidant Enzymes of Benzopyrene Mice

作　　者：钟正贤[1] 杨秀芬[2] 陈学芬[1] 李燕婧[1] 

机构地区：[1]广西中医药研究院,广西南宁530022 [2]广西中医学院药学院,广西南宁530002

出　　处：《云南中医中药杂志》2009年第7期51-52,共2页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：国家自然科学基金项目(编号:30660228)

摘　　要：目的:探讨显齿蛇葡萄叶总黄酮对苯并芘染毒小鼠血清和肝脏抗氧化酶的影响。方法:用显齿蛇葡萄叶总黄酮给小鼠灌服2周后,一次性灌胃苯并芘染毒,取血和肝脏测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH—PX)、过氧化氢酶(CAT)、r-谷氨酰转肽酶(r-GT)活性和丙二醛(MDA)含量。结果:显齿蛇葡萄叶总黄酮能明显提高小鼠血清和肝脏中SOD、GSH—PX、CAT的活性,降低r-GT活性及MDA含量。结论:显齿蛇葡萄叶总黄酮具有抗氧化作用,可能是显齿蛇葡萄叶总黄酮的抗致癌物作用机制之一。Objective : To study the effect of flavonoids of Ampelopsis grmsedntara leaves onthe serumandliver antioxidant enzymes ofbenzopyrene mice . Methods :Theflavonoids of Ampelopsis grmsedntaraleaves was usedto perfusethe micefor 2 weeks ,one-ti me perfusionof Benzopyrene administered to take the blood and liver to determine the contents of superoxide dismutase(SOD) ,glutathione peroxidase(GSH-PX) ,gatalase (CAT) ,r-glutamyltranspeptidase enzyme (r-GT) activity and malondialdehyde( MDA) . Results : The flavonoids ofampelopsis grmsedntara leaves could obviously raise the activity of SOD、GSH-PX、CATin the serumand liver of the mice and lower theactivity of r-GTand the content of MDA.Conclusion:Ampelopsis grmsedntaraleaves have antioxidation effect whichis probably one of themechanisms of anti-carcinogens of the flavonoids of Ampelopsis grmsedntara leaves .

关 键 词：显齿蛇葡萄叶总黄酮 苯并芘 抗氧化酶 小鼠 

分 类 号：R285.5[医药卫生—中药学]