成纤维细胞端粒酶重建对生物节律的调控  

Telomerase Reconstitution in Fibroblasts Regulates Cell Circadian Rhythm

作　　者：黄翔[1] 王正荣[2] 魏大鹏[2] 杨春蕾[2] 母得志[2] 屈艺[2] 

机构地区：[1]四川省人民医院外科,成都610072 [2]四川大学华西医学中心,成都610041

出　　处：《临床医学工程》2009年第7期4-5,共2页Clinical Medicine & Engineering

基　　金：四川省科技厅基金项目(项目编号:08ZQ026-069;07JY029-067);四川省卫生厅基金项目(项目编号:070164)

摘　　要：为探讨衰老对生物节律调控的分子机理,以青龄、衰老和端粒酶重建的皮肤成纤维细胞作为细胞模型,检测节律基因Per2,BMAL1和Clock表达。衰老细胞中节律基因表达受损,端粒酶重建细胞恢复了节律基因的节律性表达,提示端粒酶重建细胞可望用于衰老细胞的替代治疗。The entrainment of the circadian signals to external or suprachiasmatic nucleus stimulation in the peripheral clocks is essential for maintaining the normal function of human body. However, aging will disrupt the entrainment of peripheral circadian rhythms, thus leading to some age associated diseases. Up till now, little is known about the modification of the oscillatory rhythms in aged cells. A recent report showed that cell senescence in vascular human smooth muscle cells （HSMCs） altered circadian rhythms by a disregulation of rhythmic genes ~xpression. Furthermore, this alteration could be reversed by telomerase reconstitution. To test whether telomerase reconstitution can restore tisrupted circadian rhythm in other types of senescent cells, we used fibroblasts as cell models to profoundly investigate the relationship between cell senescence and circadian rhythm modulation. We found that the response of rhythmic genes expression to serum stimulation was markedly attenuated in senescent fibroblasts, and telomerase reconstituted fibroblasts reset the circadian oscillation of rhythmic genes exprestion. These findings suggested that telomerase reconstitution might be a good way to reset entrainment of peripheral circadian rhythms disrupted in senescent tissues.

关 键 词：端粒酶 皮肤成纤维细胞 生物节律 节律基因 

分 类 号：R339.38[医药卫生—人体生理学] R329.2[医药卫生—基础医学]