实时定量逆转录PCR分析D-半乳糖致衰老小鼠胸腺相关基因表达的研究  

Thymic-associated Genes Expression Investigation of D-galactose Induced Aging Mouse Model by Real-time RT-PCR Method

作　　者：张华华[1] 张国平[1] 

机构地区：[1]广东医学院医学遗传学研究室,广东湛江524023

出　　处：《临床医学工程》2009年第7期6-7,共2页Clinical Medicine & Engineering

基　　金：广东省湛江市科技计划项目(2007C02010);广东医学院博士启动基金(B2006097)

摘　　要：目的建立D-半乳糖致衰老小鼠模型,分析其胸腺相关基因表达的研究。方法D-Gal模型组小鼠皮下注射剂量为500mg/kg·d的D-半乳糖溶液,连续注射8w;实时定量逆转录PCR方法对D-Gal模型组小鼠的几种胸腺状态和功能相关基因的表达进行相对定量以直接评价胸腺微环境。结果D-Gal模型组与对照组相比,胸腺相关基因表达变化比值为:LMO2:0.94±0.76,Foxn1:0.45±0.32,IL-7:0.69±0.7。结论D-Gal模型组与对照组相比,胸腺负调控因子的表达无明显变化,而胸腺微环境上皮细胞和胸腺细胞分泌及必需的因子的基因表达显著下降,因而在个体水平上,D-Gal破坏了胸腺微环境的稳定性和功能性,使小鼠胸腺发生明显退化。Objective To establish D-galactose induced aging mouse model and investigate thymic-associated genes expression. Meth. ods Thymic-associated genes expression were measured by re al-time RT-PCR method valuing directly thymic microenvironment. Results Compared with control group, thymic-associated genes expression ratios of D-gal group were LMO2： 0.94±0.76, Foxnl 0.45±0.32, IL-7： 0. 69±0.7. Concusions In contrast to control group, thymus microenvironment negative gene expressions in D-Gal group have no obvious change, but some necessary gene expressions decrease. So, D-Gal treatment can induce thymus involvation.

关 键 词：D-半乳糖 胸腺 实时定量逆转录PCR 

分 类 号：R322.53[医药卫生—人体解剖和组织胚胎学]