大便潜血试验免疫法测定血红蛋白和转铁蛋白的临床应用  

Comparison of Immunoassay and TF assay in detecting fecal occult blood

作　　者：梁华英[1] 黄胜[1] 肖伟泉[1] 程洪周[1] 洪流[1] 

机构地区：[1]广东省湛江中心人民医院检验科,524037

出　　处：《检验医学与临床》2009年第14期1141-1142,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨大便潜血试验免疫法测定血红蛋白和转铁蛋白的临床应用价值。方法对60例黑色便分别使用邻联甲苯胺法、血红蛋白法和转铁蛋白法进行潜血试验检测，并对结果进行比较；另对2003-2008年先后使用上述3种方法检测的10473例随机大便标本的潜血试验结果并进行分析。结果60例黑色便潜血试验阳性率邻联甲苯胺法假阳性率16．7％，血红蛋白假阴性率10．0％，转铁蛋白法假阴性率2．0％；随机大便潜血试验阳性率邻联甲苯胺法为38．7％，血红蛋白法为11．4％，血红蛋白法与转铁蛋白法联合检测时为16．2％，3种方法差异有统计学意义（P〈0．05）。结论血红蛋白法和转铁蛋白法的灵敏度和特异性均高于邻联甲苯胺法，二者联合使用可提高检测的准确性。Objective To study the clinical value of o-tolidine assay,immunoassay and TF assay fecal occult blood tests. Methods 60 black fecal were analyzed and compared with the result by o-tolidine assay, immunoassay and TF assay on occult blood;and the result of o-tolidine, unoassay and TF assay fecal occult blood teats t were analyzed with the stochastic fecal samples between 2003--2008 year. Results Pseudo positive of fecal occult blood teats to the 60 black fecal was 100% by o-tolidine,75.0% by immunoassay and 81.7%by immunoassay connected with TF assay;Pseudo-posive of fecal occult blood teats to The stochastic fecal were 38.7 % by o-tolidine, 11.4 % by immunoassay and 16.2 by immunoassay connected with TF assay. Conclusion The sensibility and specificity of immunoassay and TF assay are higher than those of o-tolidine ,and the accuracy of the detection can be improved by the two methods .

关 键 词：粪便潜血试验 免疫法 转铁蛋白 邻联甲苯胺法 血红蛋白 

分 类 号：R446.13[医药卫生—诊断学]