黄瓜绿斑驳花叶病毒低风险参照物质的研制  

Preparation of the reference material with low biosafety risk used for molecular detection of Cucumber green mottle mosaic virus

作　　者：黄峰[1] 陈青[1] 陈红运[1] 白静[2] 李桂芬[2] 余芳平[1] 陈洪俊[2] 

机构地区：[1]厦门出入境检验检疫局,厦门361026 [2]中国检验检疫科学研究院

出　　处：《植物检疫》2009年第4期24-26,共3页Plant Quarantine

基　　金：国家科技支撑计划项目(2006BAD08A13);国家质检总局项目(2007IK253)

摘　　要：采用RT-PCR方法扩增黄瓜绿斑驳花叶病毒的外壳蛋白基因与3'非编码区,并将其构建到马铃薯X病毒(PVX)载体中。重组质粒经线性化及体外转录后接种烟草,获得含有病毒外壳蛋白基因的感病植株。感病组织可用于分子检测的质控,也可作为毒源来繁殖阳性参照物质。基于PVX载体制备的参照物质可降低检疫性病毒的生物安全风险,尤其对稳定性强、可造成严重经济损失的高风险病毒的检疫更具实用价值。The CP gene and 3＇noncoding region of Cucumber green mottle mosaic virus （CGMMV） were constructed into the PVX based virus vector p45P46. In vitro RNA transcripts from recombinant plasmid pPVXCGMMV - CP were mechanically inoculated onto Nicotiana benthamiana. The diseased plants could be utilized as virus source and the reference material for molecular detection. Preparation of the reference material of CGMMV based on PVX vector can reduce biosafety risk effectively. The method in this study provides a feasible protocol for producing reference materials of plant viruses, especially for those which were highly stable and could cause great economic losses.

关 键 词：黄瓜绿斑驳花叶病毒 参照物质 

分 类 号：S41-30[农业科学—植物保护]