整合素连接激酶在慢性移植肾肾病患者肾组织中的表达及意义  

Expression and Significance of Integrin-linked kinase in Renal Tissues of Patients with Chronic Allograft Nephropathy

作　　者：晏强[1] 解水勇[2] 睢维国[1] 邹和群[3] 

机构地区：[1]解放军181医院肾脏科,桂林541002 [2]南方医科大学,硕士研究生广州510515 [3]中山大学附属第五医院肾脏科肾脏病移植研究室,珠海519000

出　　处：《中国中西医结合肾病杂志》2009年第7期591-594,I0004,共5页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:探讨移植肾组织中整合素连接激酶(ILK)及Ⅳ型胶原的表达,及与转化生长因子-β1(TGF-β1)表达和慢性移植肾肾病(CAN)的关系。方法:用免疫组织化学技术和计算机真彩色图像分析系统半定量检测30例CAN患者移植肾组织中ILK、Ⅳ型胶原和TGF-β1的表达情况,分析三者间及与CAN病理分级之间的关系。10例正常肾组织作为对照。结果:CAN移植肾组织中ILK、Ⅳ型胶原、TGF-β1的表达比正常肾组织明显增加(P<0.001),并随CAN病理分级呈逐渐递增的趋势。移植肾组织中ILK的表达与TGF-β1、Ⅳ型胶原呈正相关(r分别为0.822、0.715,P<0.001)。结论:研究结果显示ILK可能介导TGF-β1促进CAN细胞外基质(ECM)异常沉积发病机制,Ⅳ型胶原的异常沉积是CAN患者移植肾纤维化的重要表现,且ILK在CAN移植肾纤维化进程中起重要作用。Objective：To investigate the expressions of integrin linked kinase（ILK） and collagen Ⅳ in the renal allografts of patients with chronic allograft nephropathy （CAN）, and explore their relationship with transforming growth factor -β1（TGF -β1 ） expression and the pathogene.sis of CAN. Methods： Immunohistochernical assay and computer - assisted genuine colored image analysis system were used to detect the expressions of ILK, TGF - β1 and collagen Ⅳ in the renal allografts of patients with CAN. The association between TGF-β1, collagen Ⅳ and ILK, as well as the relationship between their expressions and the pathological grading of CAN,were analyzed. Ten specimens of healthy renal tissue were used as controls. Results： The expressions levels of ILK, TGF-β1 and collagen Ⅳ were significantly higher in the renal tissues of the patients, compared to those in the normal renal tis,sues（P 〈 0. 001 ） ,and the expressions tended to increase with the pathological grades of CAN. In CAN group, the expression of ILK was positively correlated with those of TGF-β1 and collagen Ⅳ（ r = 0. 822 and r = 0.715, respectively; P 〈 0.001 for both）. Conclusion： It is indicated by the data that ILK might mediate the mechanism through which TGF-β1 promoted the abnormal deposition of ECM in renal allograft with CAN and the abnormal deposition of collagen Ⅳ in renal allograft might be important manifestation of fibrosis in CAN. It is suggested that ILK might play an important role in the progrexsion of renal fibrosis in CAN.

关 键 词：整合素连接激酶 慢性移植肾肾病 转化生长因子-Β1 

分 类 号：R692.9[医药卫生—泌尿科学]