黄瓜果实不同部位的耐冷性差异  

Changes of Chilling Tolerance in Different Sections of Cucumber Fruit

作　　者：赵宇瑛[1] 杨静[1] 陈金凤[1] 茅林春[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,杭州310029

出　　处：《园艺学报》2009年第7期1060-1064,共5页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30771513)

摘　　要：为了研究黄瓜果实采后的耐冷性,将‘津优1号’黄瓜(Cucumis sativus L.)果实在2℃下贮藏9d后,转移到20℃下放置2d,分别测定头部(花萼端)、中部、尾部(果梗端)3个部位的冷害指数、电导率、磷脂酶D(PLD)和脂氧合酶(LOX)活性、膜结合Ca2+含量。黄瓜果实的冷害最初在头部出现,再逐渐蔓延到中部和尾部。冷藏以后的黄瓜果实冷害指数、电导率、PLD活性、LOX活性均呈现为头部最高,中部次之,尾部最低。与膜结合的Ca2+含量则从黄瓜头部到尾部逐渐递增。结果表明,黄瓜果实从尾部到头部的耐低温能力逐渐降低,果实冷害程度与PLD和LOX活性提高和膜结合钙离子浓度下降显著相关。To determine the chilling tolerance of cucumber fruit after harvest, cucumber （ Cucumis sativus L. ‘Jinyou 1＇ ） fruit were stored at 2℃ for 9 days before ripening at 20℃ for 2 days. Chilling injury index, electrolyte leakage, activities of phospholipase D （PLD） and lipoxygenase （LOX）, membrane-associated Ca2＋ content in the calyx, middle and stalk sections of cucumber fruit were determined, respectively. C injury occurred initially in the calyx area, and developed toward the middle and stalk end. After storage, chilling injury index, electrolyte leakage, activities of PLD and LOX in calyx section were highest, and lowest in stalk section of the fruit. Content of membrane-associated Ca2＋ increased from calyx to stalk. Date indicated that increasing tolerance to chilling temperature from calyx to stalk of cucumber fruit was significantly related with the stimulation of PLD and LOX activities and decrease of membrane-associated Ca2＋ content.

关 键 词：黄瓜 冷害 PLD LOX 钙 

分 类 号：S642.2[农业科学—蔬菜学]