电针对β-淀粉样前体蛋白转基因小鼠行为学及其淀粉样前体蛋白、β淀粉样蛋白及胆碱乙酰转移酶水平的影响  

Effect of Electroacupuncture on the Behavior and the Expression of Amyloid β-protein,Amyloid Precursor Protein and ChAT in APP 695 V 717 I Transgenic Mice

作　　者：薛卫国[1] 张忠[1] 白丽敏[1] 许红[1] 吴海霞[1] 

机构地区：[1]北京中医药大学基础医学院人体形态系,北京100029

出　　处：《针刺研究》2009年第3期152-158,共7页Acupuncture Research

基　　金：高等学校博士学科点专项科研基金资助(No.20060026015)

摘　　要：目的:探讨针刺治疗阿尔茨海默病(AD)的可能作用机制。方法:将3月龄的β-淀粉样前体蛋白(APP)转基因小鼠随机分为模型组和电针组,每组6只,以相同月龄的C 57 BL/6 J小鼠6只为正常组。电针取"百会""涌泉"穴,每次治疗15 min,隔日1次,治疗3个月。以Y型电迷宫测定APP转基因小鼠学习记忆能力,免疫组化技术检测脑内APP、β淀粉样蛋白(Aβ)及胆碱乙酰转移酶(ChAT)水平。结果:模型组Y型电迷宫试验达标所需训练次数明显多于正常组(P<0.05),电针组达标所需训练次数较模型组明显减少(P<0.01)。模型组大脑皮层、海马CA 1区的APP阳性细胞面积及Aβ水平明显高于正常组(P<0.01),大脑皮层ChAT水平低于正常组(P<0.05);电针组大脑皮层、海马CA 1区APP阳性细胞面积及海马CA 1区Aβ水平低于模型组(P<0.05),大脑皮层、海马CA 1区ChAT水平高于模型组(P<0.05,P<0.01)。结论:电针可能通过降低APP转基因小鼠大脑皮层及海马CA 1区APP、Aβ表达水平,增加ChAT表达水平,达到改善其学习、记忆能力的作用。Objective To observe the effect of electroacupuncture （EA） on the expression of amyloid precursor protein （APP） and amyloid β-protein （Aβ） in hippocampal CA 1 area and cerebral cortex in APP 695 V 717 I transgenic mice, so as to investigate its underlying mechanism in improving Alzheimer＇s disease （AD）. Methods APP 695 V 717 I transgenic mice were randomly divided into model and EA groups, with 6 cases in each. The other 6 negatively-transgenic mice （C 57 BL/6 J） were used as the normal control group. EA （2 Hz/100 Hz, 3 mA） was applied to ＂Baihui＂ （GV 20） and ＂Yongquan＂ （KI 1） for 15 min, once every other day for 3 months. The learning-memory ability of mice was measured by Y-type maze test and the expression levels of APP, A15, and choline acetyltransferase （ChAT） in the cerebral cotex and hippocampal CA 1 area were detected by immunohistochemistry. Results In comparison with control group, the times of training for gaining correct reaction and the expression levels of Aβ and APP in both cerebral cortex and hippocampal CA 1 area in model group increased significantly （P〈0.05, P〈0.01 ）, while the expression level of ChAT of cerebral cortex in model group decreased considerably （P〈0.05）. Compared to model group, the times of training for gaining correct reaction and the expression levels of APP in both cerebral cortex and hippocampal CA 1 area, and Aβ in CA 1 area of EA group lowered remarkably （ P〈0.05）, while those of ChAT in cerebral cortex and hippocampal CA 1 area of EA group increased evidently （P〈0.05, P〈0.01 ）. Conclusion Electroacupuncture can improve the learning-memory capacity of the APP transgenic mice, which may be related to its effects in increasing the production of acetylcholine and lowering the levels of APP and Aβ in the brain.

关 键 词：阿尔茨海默病 APP转基因小鼠 电针 学习记忆 大脑皮层 海马CA1区 

分 类 号：R245.97[医药卫生—针灸推拿学]