电针对戊四唑点燃型癫痫大鼠脑电及海马神经元内Ca^(2+)含量的影响  

Effect of Electroacupuncture on Epileptic EEG and Intracellular Ca^(2+) Content in the Hippocampus in Epilepsy Rats

作　　者：杨帆[1,2] 徐国龙[3] 杨永清[1] 沈德凯[2] 冯品智[2] 王频[2] 刘向国[4] 

机构地区：[1]上海市针灸经络研究所,上海200030 [2]安徽中医学院针灸经络研究所,合肥230038 [3]安徽中医学院中医临床学院,合肥230038 [4]安徽中医学院中西医结合学院,合肥230038

出　　处：《针刺研究》2009年第3期163-166,共4页Acupuncture Research

基　　金：国家中医药管理局课题(No.02-03jp19)

摘　　要：目的:探讨电针抗癫痫的可能机制。方法:SD大鼠20只随机分为正常组、模型组、尼莫地平组、电针组。除正常组外,其它3组均制作戊四唑慢性点燃癫痫模型。电针组电针"百会""大椎",尼莫地平组腹腔注射尼莫地平。记录各组大鼠治疗后痫样波发作潜伏期及痫波密度,使用激光共聚焦显微镜观察大鼠海马神经元内Ca2+的荧光强度。结果:电针组脑电痫样波发作潜伏期较模型组明显延长(P<0.05),痫波密度与模型组比较明显变小(P<0.05)。模型组大鼠海马神经元Ca2+荧光强度较正常组明显升高(P<0.01),电针组Ca2+荧光强度较模型组明显降低(P<0.05),尼莫地平组Ca2+荧光强度较模型组明显降低(P<0.01)。结论:电针可调节戊四唑点燃型癫痫大鼠脑电及海马内Ca2+含量,电针的抗癫痫作用可能与之有关。Objective To study the underlying mechanism of electroacupuncture （EA） in relieving epilepsy in pentylenetetrazole （PTZ）-induced epilepsy rats. Methods Twenty SD rats were randomly divided into normal control, model, EA, Nimodipine groups, with 5 cases in each. Epilepsy model was established by intraperitoneal injection of PTZ （32.0 mg/kg）, once daily for 28 days. EA （100 Hz, 0.6 mA） was applied to ＂Baihui＂（GV 20） and ＂Dazhui＂（GV 14）, once daily for 7 days. For Nimodipine group, the rats were given with nimodipine （0.25 mg/kg, i. p. ）, once daily for 7 days. Electroencephalogram （EEG） was recorded and the fluorescence intensity of Ca^2＋ of the hippocampus tissue sections was detected by laser scanning confocal microscope （LSCM） after incubation in artificial cerebrospinal fluid containing Flou-3/AM （10 μmol/L） and pluronic F-127 （5 μl）. Results Compared with model group, the latencies of epileptic EEG seizure prolonged obviously （P〈0, 05）, and epileptic EEG seizure frequencies decreased significantly （P〈0.05） in EA and Nimodipine groups. The fluorescence intensity of intracellular Ca^2＋ in hippocampus tissue in model group was obviously higher than that in control group （P〈0.01）. In comparison to model group, Ca^2＋ levels in EA and Nimodipine groups lowered considerably （P〈0.05, P〈0.01 ）. No significant differences were found between EA and Nimodipine groups in the aforementioned 3 indexes （P〉0.05）. Conclusion EA has an obvious anti-epileptic effect, which may be closely related to its effect in downregulating the increased hJppocampal Ca^2＋ level in PTZ-kindled epilepsy rats.

关 键 词：癫痫 电针 脑电图 CA^2+ 海马 

分 类 号：R245.97[医药卫生—针灸推拿学]