电针对心肌缺血模型大鼠孤束核c-fos表达及心电图ST_(Ⅱ)的影响  

Effects of Electroacupuncture on c-fos Expression in Nucleus of the Solitary Tract and Electrocardiogram ST_(Ⅱ) in Myocardial Ischemia Rats

作　　者：李江山[1] 严洁[1] 何军锋[1] 彭娜[1] 

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410208

出　　处：《针刺研究》2009年第3期171-174,共4页Acupuncture Research

基　　金：湖南省教育厅青年基金课题(No.04B047)

摘　　要：目的:观察针刺"内关""足三里"对心肌缺血模型大鼠孤束核(nucleus of the solitary tract,NTS)c-fos表达及心电图STⅡ的影响,阐明延髓初级中枢在"内关""足三里"穴共同调节心功能中的作用。方法:将36只雄性SD大鼠随机分为生理盐水组、模型组、足三里组、内关组、偏历组及合阳组,每组6只,腹腔注射异丙肾上腺素造成心肌缺血模型。电针治疗20 min,采用抗Fos蛋白免疫组织化学技术观察大鼠NTS内c-fos表达和电生理技术观察大鼠心电图STⅡ的变化。结果:心肌缺血模型大鼠NTS内c-fos的表达明显增加(P<0.01),而足三里组、内关组与模型组比较,NTS内c-fos阳性细胞数均明显减少(P<0.01);足三里组、内关组NTS内c-fos阳性细胞数显著低于偏历组、合阳组(P<0.01)。足三里组、内关组大鼠缺血性心电图STⅡ电位值显著降低(P<0.01),其电位降低值大于偏历组、合阳组(P<0.01)。结论:NTS是针刺内关、足三里共同调节心功能的整合中枢之一。Objective To observe the effect of electroacupuncture （EA） of ＂Neiguan＂ （PC 6） and ＂Zusanli＂ （ST 36） on c-fos expression in the nucleus of the solitary tract （NTS）and ischemic electrocardiogram （ECG） in acute myocardial ischemia （AMI） rats, so as to study the role of NTS in EA-induced improvement of AMI. Methods Thirty-six male SD rats were randomly divided into control, model, ST 36, PC 6, ＂Pianli＂ （LI 6）, and ＂Heyang＂ （BL 55） groups, with 6 cases in each. AMI model was established by intraperitoneal injection of Isoproterenol （5 mg/kg）. EA （4 Hz/20 Hz, 1 mA） was applied to PC 6, ST 36, LI 6 and BL 55 for 20 min separately, c-fos expression in the NTS was detected by immunohistochemistry and ECG-STⅡ was recorded before and after AMI and EA. Results ① Compared to control group, ECG-STⅡ of model group elevated significantly, while in comparison with model group, ECG-STⅡ values of ST 36 and PC 6 groups decreased significantly （P〈0.01）, suggesting an improvement of AMI after EA. No significant changes of ECG-STⅡ were found in LI 6 and BL 55 groups （P〉0.05）. ② The number of fos-immunoreaction （fos-IR） positive neurons in the NTS in ST 36 and PC 6 groups was significantly lower than that in model, LI 6 and BL 55 groups （P〈0.01）. Conclusion EA of PC 6 and ST 36 can improve ischemic ECG in AMI rats and NTS is one of the centers for integrating cardiac functional activity and information from EA of PC 6 and ST 36.

关 键 词：急性心肌缺血 电针 内关 足三里 孤束核 C-FOS表达 

分 类 号：R245.97[医药卫生—针灸推拿学]