Cx40、43调节缺氧处理大鼠肠系膜上动脉收缩反应性的信号途径  

Signal pathway of Cx40/43 regulates vascular contractile reactivity of superior mesenteric artery under hypoxic condition

作　　者：明佳[1] 李涛[1] 杨光明[1] 徐竞[1] 张瑗[1] 刘良明[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第二研究室,创伤,烧伤与复合伤国家重点实验室,重庆400042

出　　处：《中国病理生理杂志》2009年第7期1262-1265,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30625037);国家"973"计划资助项目(No.2005CB522601);国家自然科学基金重大资助项目(No.30830053);重庆市自然科学基金资助项目(No.2008BB5102)

摘　　要：目的:研究连接蛋白(connexin,Cx)40或43对cAMP-PKA、cGMP-PKG和DG-PKC信号通路的影响及对缺氧处理大鼠肠系膜上动脉内膜依赖的血管收缩反应性的调节作用。方法:以SD大鼠肠系膜上动脉(superior mesenteric artery,SMA)为研究对象,用Cx40或Cx43的反义寡脱氧核苷酸(antisense oligodeoxyribonucleoti-de,AODN)阻断SMA的Cx40或Cx43的表达,观察缺氧处理后血管的环一磷酸腺苷(cyclicadenosinemonophos-phate,cAMP)、环一磷酸鸟苷(cyclic guanosine monophosphate,cGMP)、二酰基甘油(diacylglycerol,DG)浓度和蛋白激酶A(protein kinase A,PKA)、蛋白激酶G(protein kinase G,PKG)、蛋白激酶C(protein kinase C,PKC)活性的变化,以及这些变化与内膜依赖的血管收缩反应性变化的关系。结果:Cx40AODN可以降低血管cAMP、cGMP的浓度和PKA、PKG的活性,增加DG的浓度、PKC的活性和血管内膜依赖的收缩反应性;Cx43AODN可以增加血管cAMP、cGMP的浓度和PKA、PKG的活性,降低DG的浓度、PKC的活性和血管内膜依赖的收缩反应性。结论:Cx40、Cx43通过cAMP-PKA、cGMP-PKG、DG-PKC信号通路参与了休克后内膜依赖的血管收缩反应性的调节。AIM ： To investigate the effect of connexin 40/43 （ Cx40/43 ） on cyclic adenosine monophosphate - protein kinase A （ cAMP - PKA）, cyclic guanosine monophosphate - protein kinase G （ cGMP - PKG） and diacylglycerol - protein kinase C （ DG - PKC） signal pathways and the regulatory role on the vascular contractile reactivity. METHODS： Rat superior mesenteric arteries （SMA） were isolated. The vascular rings of SMA were prepared and treated with Cx40/43 antisense oligodeoxyribonucleotide （Cx40/43AODN）. The changes of the concentration of cAMP, cGMP and DG, the activity of PKA, PKG and PKC, the contractile response of hypoxia treated SMA rings were observed. RESULTS ： Cx40AODN decreased the concentrations of cAMP, cGMP and the activities of PKA and PKG, increased the level of DA, the activity of PKC and the endothelium- dependent contractile response in SMA. Cx43AODN increased the concentrations of cAMP, cGMP and the activities of PKA and PKG, decreased the level of DA, the activity of PKC and the endothelium - dependent contractile response in SMA. CONCLUSION： Cx40/43 regulates endothelium - dependent vasoconstrictor reactivity of SMA after hemorrhagic shock, which may be related to cAMP - PKA, cGMP - PKG and DG - PKC signal pathways.

关 键 词：血管低反应性 连接蛋白40 连接蛋白43 信号通路 

分 类 号：R641[医药卫生—外科学]