小神经胶质细胞移植对脑梗死的治疗作用  

Effects of microglia transplantation on treating cerebral infarction

作　　者：张剑[1] 张敏[2] 

机构地区：[1]绍兴文理学院医学院药理研究室,浙江绍兴312000 [2]绍兴文理学院附属医院,浙江绍兴312000

出　　处：《中国病理生理杂志》2009年第7期1331-1335,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30800360);国家人事部留学人员科技活动项目择优资助计划资助项目;浙江省自然科学基金资助项目(No.Y204364);浙江省教育厅资助项目(No.20061164)

摘　　要：目的:探讨小神经胶质细胞(MG)移植对脑梗死的治疗作用及其机制。方法:采用光化学局灶性脑梗死大鼠动物模型,运用组织化学、免疫组织化学方法研究经锁骨下动脉移植入MG对梗死周边区的影响。并通过免疫荧光双标和Western blotting检测MG中神经生长因子(NGF)和白细胞介素-10(IL-10)的表达。结果:脑梗死灶的周围发现大量FITC绿色荧光标记的移植而来的MG;MG移植明显减小脑梗死灶体积、减少神经元凋亡;移植而来的MG中NGF和IL-10的表达显著增加。结论:MG能够通过血脑屏障(BBB)并游走至损伤部位,并通过分泌NGF和IL-10对脑血栓脑梗死灶的周围区产生保护作用,提示MG移植可能用于脑血栓的治疗。AIM： To explore the regulating mechanism of microglia transplantation in treating cerebral infarction. METHODS： Focal cerebral infarction model was established by photochemistry. The histological and immunohistochemical methods were used to observe the effects of microglia transplantation on cerebral infarction. The expressions of nerve growth factor（NGF） and interleukin - 10（IL - 10） were detected by double fluorescent immunohistochemistry and Western blotting. RESULTS： Microglia marked with green fluorescence was observed in ischemic penumbra, indicating that microglia can be transplanted across the blood - brain barrier. Infarct volumes and death number of cells were significantly reduced compared to non - transplantation animals. The expressions of NGF and IL - 10 were markedly increased in microglia in transplantation group. CONCLUSION ： Microglia can be transplanted across the blood - brain barrier. The cells have protective effects in ischemic penumbra by secreting NGF and IL - 10, which might serve as therapeutical method for treating cerebral infarction.

关 键 词：脑梗死 小神经胶质细胞 血脑屏障 神经生长因子 白细胞介素10 

分 类 号：R332.81[医药卫生—人体生理学]