Galectin-3在雌性小鼠生殖系统中的表达及脂多糖的影响  

Galectin-3 expression in normal and LPS-stimulated genital system of female mouse

作　　者：禹艳红[1] 潘善培[1] 谢琪璇[1] 关艺青[1] 黄丹[1] 

机构地区：[1]暨南大学生殖免疫研究所,广东广州510632

出　　处：《中国病理生理杂志》2009年第7期1409-1414,共6页Chinese Journal of Pathophysiology

基　　金：中国博士后科学基金资助项目(No.200801259);广东省自然科学基金博士启动项目(No.8451063201000100)

摘　　要：目的:研究galectin-3在雌性小鼠生殖系统的表达情况,利用小鼠生殖道感染模型研究galectin-3在女性生殖道感染过程中的可能作用。方法:采用RT-PCR的方法初步鉴定小鼠生殖系统各组织中的galectin-3 mRNA的表达水平,通过阴道黏膜注射LPS不完全弗氏佐剂乳液建立小鼠的生殖道感染模型,免疫组化对ga-lectin-3蛋白在雌性小鼠生殖系统的表达进行定位分析。结果:在正常小鼠的子宫、阴道、输卵管及卵巢中都能够检测到galectin-3 mRNA的表达。Galectin-3蛋白主要表达在生殖道黏膜的上皮细胞表面和子宫腺。经LPS刺激后,galectin-3蛋白在阴道、子宫颈及子宫体的表达水平提高(P<0.05),尤其是刺激后24h子宫颈中galectin-3的表达量增加最多(P<0.01)。结论:LPS刺激能增加小鼠生殖道中galectin-3的表达,galectin-3在生殖道抗感染中可能发挥重要的作用。AIM： To study the expressions of galectin -3 and the possible anti -infectious function in genital tract of female mouse. METHODS ： The expressions of galectin - 3 mRNA in different reproductive tissues were analyzed by RT - PCR. The mouse model of genital tract infection was constructed by injection of LPS and Freunds adjuvant incomplete into the vaginal mucous. The expression pattern of galectin -3 protein was detected by immunohistochemistry. RESULTS： The expression of galectin - 3 mRNA was detected in the uterus, vagina, oviduct and ovary of normal adult mouse. The galectin - 3 protein was expressed in the genital tract mucous of epithelial cell surface and uterine gland. After stimulation with LPS, the expression of galectin - 3 protein was significantly up - regulated compared to normal control and adjuvant control （ P 〈 0. 05 ）, especially in cervix 24 h after LPS injection （ P 〈 0. 01 ）. CONCLUSION ： Stimulation with LPS up - regulates the expression of galectin - 3 in genital tract and galectin - 3 may play an important role of anti - infection in female genital tract.

关 键 词：半乳糖凝集素3 生殖道 脂多糖类 

分 类 号：R711[医药卫生—妇产科学]