玉米类受体蛋白激酶基因ZmLRRPK1的cDNA克隆与表达分析  

cDNA Cloning of Maize Protein Kinase Gene ZmLRRPK1 and Its Expression Analysis

作　　者：边鸣镝[1] 邓川[1] 白忠义[1] 

机构地区：[1]吉林大学植物科学学院,长春130062

出　　处：《中国生物工程杂志》2009年第7期33-36,共4页China Biotechnology

摘　　要：富亮氨酸重复区的类受体蛋白激酶(LRR-RLKs)在植物对多种信号的响应中发挥作用。应用电子克隆的方法在玉米中克隆了一个LRR-RLKs类基因的全长cDNA,命名为ZmLRRPK1(GenBank接受号为EU873320)。推断的ZmLRRPK1蛋白含有594个氨基酸,分子量为66kDa,等电点pI为5.42,具有典型的LRR-RLKs结构域。半定量RT-PCR结果表明,在ABA、甘露醇和氯化钠的诱导下,ZmLRRPK1在玉米胚芽鞘中表达并在24h内保持较高的转录水平。Abstract LRR-RLKs plays an important role in multiple signal transduction in plant. One fulllength cDNA encoding a LRR-RLKs homologue was isolated from maize through in silico cloning and named as ZmLRRPK1 （ GenBank accession：EU873320 ）. The predicted ZmLRRPK1 protein has 594 amino acids with an estimated molecular mass of 66kDa and an isoelectric point of 5.42. ZmLRRPK1 has the typical domain of LRR -RLKs. RT-PCR analysis indicated that ZmLRRPK1 expression was induced by ABA, mannitol and salt in coleoptiles of maize and kept high level during 24 hours.

关 键 词：玉米 富亮氨酸重复区的类受体蛋白激酶(LRR—RLKs) 信号传导 非生物胁迫 

分 类 号：Q786[生物学—分子生物学]