包涵体蛋白的分离和色谱法体外复性纯化研究进展  

The Progress of Inclusion Body Isolation and Chromatographic Refolding,Purification Methods

作　　者：王增[1] 马会勤[1] 张文[1] 陈尚武[2] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国生物工程杂志》2009年第7期102-107,共6页China Biotechnology

基　　金：国家自然科学基金资助项目(30500347&30471212)

摘　　要：重组蛋白在大肠杆菌中表达多为无活性的包涵体形式,须经洗涤、溶解、复性后才能得到生物活性蛋白。综述了近年来包涵体蛋白分离纯化和复性技术研究进展,重点讨论了色谱法复性技术的应用,包括尺寸排阻色谱、亲和色谱、离子交换色谱、疏水相互作用色谱、固定化脂质体色谱、扩张床吸附色谱的进展情况。Expression of recombinant proteins in Escherichia coli often results inclusion bodies, an aggregation form of inactive proteins. Functional proteins can only be gained through a series of steps including washing, solubilization and refolding. The inclusion body purification strategy and chromatographic refolding techniques including size exclusion chromatography, affinity chromatography, ion-exchange chromatography, hydrophobic interaction chromatography, immobilized liposome chromatography, and expanded bed adsorption chromatography were summarized in this review.

关 键 词：包涵体 纯化 色谱 复性 

分 类 号：Q939.4[生物学—微生物学] Q51