斯里兰卡肉桂碱受体在不同鼠龄大鼠耳蜗中的表达差异  

Differential Expression of Ryanodine Receptors in the Developing Rat Cochlea

作　　者：梁玉芳[1] 杨军[1] 

机构地区：[1]上海交通大学医学院附属新华医院耳鼻咽喉-头颈外科,上海200092

出　　处：《听力学及言语疾病杂志》2009年第4期358-362,共5页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金资助(30772396)

摘　　要：目的研究不同鼠龄大鼠耳蜗内斯里兰卡肉桂碱受体(ryanodine receptor,RyR)的差异表达,探讨RyR表达与耳蜗发育成熟的关系。方法分别选取出生后1天(P1)、5天(P5)、10天(P10)、14天(P14)、28天(P28)以及成年SD大鼠(>2月龄)各5只耳蜗作冰冻切片,运用免疫荧光的方法,比较各组大鼠耳蜗组织RyR的表达差异。结果P1组和P5组大鼠耳蜗Corti器内RyR表达不明显,P10组出现的RyR染色均匀,而P14组大鼠耳蜗Corti器内RyR的表达出现明显特征性变化,毛细胞突触区RyR强表达,而支持细胞内的表达相对较为广泛。大鼠出生后耳蜗螺旋神经元内RyR的表达由广泛的胞内表达,逐渐趋近细胞膜突触区。结论RyR在大鼠出生后14天表达基本成熟,与耳蜗的发育成熟基本保持一致。感觉毛细胞和螺旋神经元的RyR表达可能与神经传递等功能相关。在发育早期的螺旋神经元细胞中RyR的广泛表达,可能参与了螺旋神经元发育成熟中的细胞凋亡过程。Objective Ryanodine receptor（RyR） is one of the Ca^2＋ release channels on the intracellular Ca2＋ stores.RyR induced-Ca^2＋ release is activated by the voltage-dependent Ca2＋ entry,that is,calcium-induced calcium release（CICR）.Intracellular free Ca^2＋ concentration（i） plays a key role on cochlear function.Our study is to investigate the differential expression of RyR in the developing rat cochlea,and to analyze the relationship between the expression of RyR and auditory functional development.Methods Immature SD rats,which were 1 day（P1）,5 days（P5）,10 days（P10）,14 days（P14）,28 days（P28） after parturition,and adutl rats（5 rats for each age） were included in the study.Frozen cochlea sectioning and immunofluorescence were applied to observe the differential expression of RyR.Results The RyR expression in the Corti＇s organ increased during the cochlear development.It＇s not significant that the stain was observed on the hair cells and supporting cells in the Corti＇s organ of P1 and P5 rats.The appearance of the Corti＇s organ of P10 rats trended to maturity.In P14 rats the RyR expression on hair cells located in the synaptic area against the afferent or efferent nerve,and the strain on supporting cells was extensive.There was little different between the strain on cochlea of P14 and P28 rats.In postmature rat spiral ganglion neurons（SGN）,the RyR expression verged gradually from extensive whole cell soma to the synaptic area near to the plasma membrane.Conclusion The RyR expression peaked the 14th day after parturition,which was close to that in the mature cochlea.The time course of the RyR expression during the cochlear development was coincident with that of the auditory functional development.The RyR expression on both hair cells and SGNs located in the synaptic area near to the plasmolemma,implying that RyR induced-CICR was related to the auditory functions such as neurotransmission.Extensive RyR expression in the soma of SGNs at the early stage possibly involv

关 键 词：斯里兰卡肉桂碱受体 耳蜗发育 受体表达 钙诱导钙释放 钙 

分 类 号：R339.16[医药卫生—人体生理学]