外界生态因子对感染Wolbachia的松毛虫赤眼蜂生殖稳定性影响  

Effects of Environmental Factors on Stability of Thelytoky of Trichogramma dendrolimi Infected with Wolbachia

作　　者：张海燕[1] 张莹[1] 丛斌[1] 钱海涛[1] 董辉[1] 付海滨[2] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]沈阳出入境检验检疫局,沈阳110016

出　　处：《中国农业科学》2009年第7期2366-2372,共7页Scientia Agricultura Sinica

基　　金：国家“十一五”科技支撑计划(2006BAD08A06);国家自然科学基金项目(30771455);博士点基金项目(20040157006)

摘　　要：【目的】明确水平人工转染沃尔巴克氏体(Wolbachia)获得营产雌孤雌生殖松毛虫赤眼蜂生殖方式在环境条件改变时的遗传稳定性,探索Wolbachia与其宿主间的相互适应性。【方法】采用喂食抗生素、持续高温处理、与雄蜂交配等方法,观察产雌孤雌松毛虫赤眼蜂寄生和羽化等情况。【结果】抗生素诱导下,从F2代开始出现雄蜂,经诱导获得的雄性与正常雌性、诱导获得雌性交配可以产生后代,且20个后代群体都有雄性个体出现;25℃恒温下,与雄性交配的产雌孤雌松毛虫赤眼蜂寄生数和未交配的相比显著降低,到F7代恢复正常,只有交配的F2、F4后代出现个别雄蜂,其它世代和未交配所有世代均未出现雄蜂。而32℃高温下,交配与未交配至F5代所有羽化个体全为雄蜂。【结论】不同温度下与雄蜂交配不会改变Wolbachia感染引起的松毛虫赤眼蜂产雌孤雌生殖方式,抗生素处理和持续至少20d的32℃高温是改变感染Wolbachia的松毛虫赤眼蜂生殖方式的决定因素。[ Objective ] To clarify the genetic stability of thelytokous line of Trichogramma dendrolimi （Matsumura） infected with Wolbachia by horizontal transfer under abnormal circumstance, and adaptation between Wolbachia and its host. [Method] Parasitic, reproductive and several other biological characteristics of thelytokous line of Trichogramma dendrolimi were investigated in treatments of feeding with tetracycline, continuance of high temperature and mating. [Result] After treatment with tetracycline, male Trichogramma emerged in the F2 generation of the thelytokous line, the males induced by tetracycline mated with females induced to produce offspring, and male individuals appeared in all 20 descendant families. At normal temperature of 25℃, the mating of thelytokous line of Trichogramma dendrolimi reduced the number of parasitic compared to non-mating markedly until the F7 generation. However individual males presented incidentally in generations F2 and F4, not found in other generation with mating and all generation without mating. At relative higher temperature of 32℃, individual males presented in whole generation of F5 including mating and non-mating. [Conclusion] Stability of thelytokous line of Trichogramma dendrolimi infected with Wolbachia can not be affected by mating at 25℃ and 32℃, antibiotic treatment and higher temperature of 32℃ lasting at least 20 d are the key factors to eliminate Wolbachia to alter thelytokous of Trichogramma dendrolimi.

关 键 词：WOLBACHIA 松毛虫赤眼蜂 孤雌生殖 稳定性 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]