主动免疫抑制素提高奶牛超数排卵胚胎产量的研究  

Enhancing Embryo Yield in Superovulated Holstein Heifers by Immunization Against Inhibin

作　　者：李超[1] 梅承[1,2] 朱玉林 薛建华 钟寿坤[2] 麻柱 张胜利 施振旦[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642 [2]北京奶牛中心,北京100085 [3]广州市华美牛奶公司,广州510663

出　　处：《中国农业科学》2009年第7期2624-2628,共5页Scientia Agricultura Sinica

基　　金：国家“十一五”科技支撑奶业专项项目(2006BAB04A02-7);广州市科技局研究项目(2003U12CA011)

摘　　要：【目的】研究免疫抑制素提高奶牛超数排卵的效率以提高胚胎产量。【方法】免疫组首次免疫抑制素α亚基融合蛋白(1mg·头-1)与白油佐剂混合乳液,首免后第28、56d分别进行2次加强免疫,剂量为0.5mg·头-1。对照组同时注射生理盐水与白油佐剂的混合乳液。在第二次加强免疫后10d,采用FSH四日递减法进行超排处理,并根据母牛发情表现进行人工授精,试验一使用常规冷冻精液输精,试验二使用分离的雌性精子输精,输精后第7天进行冲胚。【结果】主动免疫抑制素能够有效提高血液抗抑制素抗体效价。试验一,免疫抑制素组头均获胚/卵总数为(15.8±2.8)枚,显著高于对照组(8.3±1.5)枚(P<0.05);头均可移植胚胎数为(9.6±3.1)枚,稍高于对照组的(5.8±1.6)枚(P>0.05)。试验二,免疫抑制素组发情时间早于对照组(P<0.05);输精后第7天血液孕酮浓度显著高于对照组(P<0.05);头均获胚胎总数和头均可移植胚胎数分别为(15.4±1.9)枚和(5.7±0.7)枚,显著高于对照组的(9.1±1.2)枚和(3.1±0.5)枚(P<0.05);免疫组获得的一级胚胎为(3.8±1.0)枚,占总可移植胚数的58%,显著高于对照组的(0.6±0.2)枚或19.8%(P<0.05)。【结论】主动免疫抑制素能够促进牛卵泡发育、提高超排效率和胚胎产量,在使用性控精液输精时不仅大幅提高雌性胚胎数量,同时大幅提高胚胎质量。[Objective] This study aimed to increase embryo production in superovulated heifers by immunization against inhibin. [Method] Immunization procedures consisted of primary injection （i.m.） of lmg recombinant inhibin a-subunit protein in 1 ml mineral oil adjuvant, and two booster immunizations with 0.5 mg inhibin protein at 28 day intervals. Ten days after the 2nd booster immunization, all animals were superovulated with exogenous FSH, inseminated either with conventional semen （Experiment 1） or sex sorted female semen （Experiment 2）. The embryos were collected and evaluated 7 days after insemination. [Result] Immunization significantly increased blood antibody titres against recombinant inhibin a subunit protein （P〈0.01）. In experiment 1, the immunized heifers produced more embryos and ova than the control heifers （15.8±2.8 vs 8.3±1.5, P〈0.05）, and non-significantly more number of transferable embryos （9.6±3.1 vs 5.8±1.6, P 〉 0.05）. In experiment 2, inhibin immunized heifers initiated estrus earlier after FSH treatment, and had higher plasma progesterone concentrations on the day of embryo collection. In the inhibin immunized heifers, the total number of embryo/ova were （15.4±1.9）, significantly higher than that （9.1±1.2） of control heifers （P〈0.05）, while the total number of transferable embryo in the former （5.7±0.7） was also higher than in the latter group （3.1±0.5） （P 〈0.05）. Within the transferable embryos, there was （3.8±1.0） or 58% grade 1 embryos in the immunized heifers, higher than the 0.6±0.2 or 19.8% in control heifers （P〈0.05）. [Conclusion] These results indicated that immunization against inhibin increased heifer response to superovulation, and led to higher yield of total and transferable embryos, as well as increased the embryo quality.

关 键 词：奶牛 免疫抑制素 超数排卵 胚胎产量 

分 类 号：S823[农业科学—畜牧学]