百合种质资源遗传多样性及亲缘关系的RAPD分析  

Genetic Diversity and Phylogenetic Relationship Analysis on Germplasm Resources of Lily by RAPD

作　　者：童巧珍[1,2] 周日宝[2] 盛孝邦[1] 刘湘丹[2] 王朝晖[2] 刘笑蓉[2] 谭周进[2] 

机构地区：[1]湖南农业大学,湖南长沙410128 [2]湖南中医药大学,湖南长沙410208

出　　处：《农业现代化研究》2009年第4期509-512,共4页Research of Agricultural Modernization

基　　金：国家科技支撑项目"大宗道地中药材玉竹和百合规范化生产关键技术研究"(2006BAI06A11-06);湖南省中医药管理局资助项目"湖南省药食两用百合种内居群的多样性研究及应用"(2008089)

摘　　要：以百合新鲜鳞叶为材料,利用RAPD分子标记技术对16份不同生态型的百合种质的遗传多样性和亲缘关系进行了分析。研究结果表明,从120条随机引物中筛选出35条有效引物,共得到769个扩增位点,其中628个位点具有多态性,占81.7%。UPGMA聚类分析结果表明,16份百合种质在阈值为0.9407处分为2个聚类群,即百合科两个不同属的植物:百合属和大百合属;阈值为0.5790处,百合属被分为3个不同的种:百合、卷丹和细叶百合。聚类结果和亲缘关系分析表明不同供试百合之间的遗传多样性较高,亲缘关系较远,且各种质遗传距离与地理距离具有一定的相关性。In this study, the genetic diversity and phylogenetic relationship among 16 genotypes of Lily collected from different ecological sources was analyzed by RAPD. Thirty-five available RAPD primers were screened from 120 random primers, and a total of 769 DNA bands were amplified, in which 628 bands were polymorphic, the percentage is 81.7%. Cluster analysis showed that the 16 Lily germplasms were divided into two groups at 0.9407. Corresponding to two genus in liliaceae： Lilium and Cardiocrinum, and that germplasms in Lilium were further divided into three different types at 0.5790： Lilium brownii F.E. Brown var.viridulum Baker, L. lancifolium Thunb and L. pumilum DC. Results suggested that the genetic diversity is very high among different Lily germplasms and the genetic distance among germplasms was relative to their geographic distance.

关 键 词：百合 种质资源 RAPD 聚类分析 

分 类 号：S644.1[农业科学—蔬菜学]