高效液相色谱法测定川芎不同炮制品中游离阿魏酸和总阿魏酸的含量  

Determination of Free Ferulic Acid and Total Ferulic Acid in Different Processed Products of Rhizoma Chuanxiong by HPLC

作　　者：夏荃 文惠玲[1] 李土光[1] 贺洁儿[1] 

机构地区：[1]广州中医药大学中药炮制教研室,广东广州510006

出　　处：《广州中医药大学学报》2009年第4期384-387,共4页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】测定并比较川芎不同炮制品中游离阿魏酸、总阿魏酸的含量。【方法】采用超声提取法,以甲醇—甲酸(95∶5,V/V)为游离阿魏酸的提取溶剂,以甲醇—20 g/L NaHCO3(95∶5,V/V)为总阿魏酸的提取溶剂;采用高效液相色谱(HPLC)法测定,色谱条件为:Diamonsil C18分析柱(250 mm×4.6 mm,5μm);流动相为乙腈—体积分数1%冰醋酸(20∶80,V/V);检测波长:320 nm;流速:1.0 mL/min;柱温:室温。【结果】酒炙川芎的游离阿魏酸和总阿魏酸含量均高于生品及制川芎。川芎生品与不同制品水煎液中阿魏酸含量均高于其游离阿魏酸含量。【结论】本研究含量测定方法简便、准确,可用于川芎及其炮制品中阿魏酸含量的测定研究;测定川芎中总阿魏酸的含量更能反映川芎的质量。Objective To determine the contents of free ferulic acid and total ferulic acid in different processed products of Rhizoma Chuanxiong. Methods With methanol-formic acid （95：5 ） as the extraction solvent for free ferulic acid and with methanol -2% NaHCO3 water solution （95：5 ） as the extraction solvent for total ferulic acid, ultrasonic method was used for the extraction. The contents of free ferulic acid and total ferulic acid were determined by HPLC, and the chromatographic conditions were as follows： C18 column （250mm ×4.6mm, 5μm）, the mobile phase consisting of acetonitrile-1% acetic acid solution （20： 80）, the detecting wavelength at 320nm, flow rate being 1.0 mL/min and detection at room temperature. Results The average contents of free ferulic acid and total ferulic acid in Rhizoma Chuanxiong roasted by wine were higher than those in raw and other processed Rhizoma Chuanxiong, and free ferulic acid content in raw and different processed Rhizoma Chuanxiong was lower than the total ferulic acid content. Conclusion The method is simple, accurate and can be used for the quality control standard for Rhizoma Chuanxiong, and the chemical assay of total ferulic acid content would be a better choice of assessing the herbal quality of Rhizoma Chuanxiong.

关 键 词：川芎/生产与制备 川芎/化学 阿魏酸/分析 色谱法 高压液相 

分 类 号：R284.1[医药卫生—中药学]