硫酸盐还原菌对多种染料厌氧脱色的特性  

The Properties of Decolorization of Dyes by SulfateReducing Bacteria

作　　者：贾省芬[1] 杨惠芳[1] 刘双江[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《环境科学》1998年第4期18-21,共4页Environmental Science

摘　　要：研究专性厌氧菌硫酸盐还原菌混合培养物对偶氮染料、三苯甲烷染料、蒽醌染料的脱色作用．在菌的生长过程中，随着硫化氢的形成，染料的脱色率不断增长．分别测定了完整细胞和上清液对５种染料的脱色能力．当上清液中含有硫化氢时，在１ｈ内不同染料（５０ｍｇ／Ｌ）的脱色率可达２５．５８％—９８．８％；而完整细胞的脱色率则为６．９９％—８０．６０％．由此表明，在厌氧硫酸盐还原条件下，硫酸盐还原菌及其产物硫化氢对染料的脱色均有重要作用．完整细胞对１０种结构不同的染料（５０ｍｇ／Ｌ）的半脱色时间为０．５—３２．６ｈ，甚至更长，脱色的最适温度和ｐＨ分别为３０℃和８．０，严格厌氧；还原型烟酰胺腺嘌呤二核苷酸对脱色具有促进作用，粗酶液对酸性红Ｂ２ＧＬ脱色比活（染料／蛋白）为０．８７μｇ／（ｍｇｍｉｎ）。The decolorization of azo dyes,tribenzic methane dyes and anthracene dyes by anaerobic bacteria,sulfatereducing bacteria(SRB) were studied in this paper.In the anaerobic growth of SRB,the decolorization rates of dyes increased with the forming of H2S.The decolorizing abilities of intact cells and supernatant for 5 dyes were also determined,the results showed that the decolorization rates of these dyes(50mg/L) by supernatant containing H2S were 2558%—988% within reaction for 1h,and those by intact cells were 699%—8060%.It was found that both of cells and H2S producing from the bacteria acted important roles in the decolorization of dyes.The half decolorization times for 10 different dyes by intact cells lay from 05 to 326h or more.The optimum temperature and pH were 30℃ and 80,respectively.Reduced nicotinamide adenine dinucleotide(NADH) could improve the decolorizing activities.The specific activity and MichaclisMenten Constant(Km) value of crude enzyme for Acid Red B2GL were 087μg/(mg·min) and 14mmol/L,respectively.

关 键 词：硫酸盐还原菌 染料 厌氧脱色 废水处理 脱色 

分 类 号：X791.03[环境科学与工程—环境工程]