克菌丹的亚慢性经口毒性研究  

Research on the sub-chronic oral toxicity of captan

作　　者：阙冰玲[1] 葛怡琛[1] 梁丽燕[1] 黄建勋[1] 张子群[1] 

机构地区：[1]广东省职业病防治院,广东广州510300

出　　处：《中国职业医学》2009年第3期193-195,共3页China Occupational Medicine

摘　　要：目的研究克菌丹的亚慢性经口毒性,确定克菌丹亚慢性经口的最大无作用剂量并初步确定毒作用靶器官。方法96只SD大鼠分为4组,每组24只,分别为空白对照组,受试物低、中、高剂量组,共4个实验组,分别用含0、333、1105和3333mg/kg克菌丹的饲料给无特定病原体级大鼠喂饲90d(动物实际摄食克菌丹的量在雌性分别为0、36.0、124.4、371.3mg·kg-1·d-1,在雄性分别为0、30.9、94.2、323.6mg·kg-1·d-1)。以一般状态、体质量、血常规9项,血生化12项,尿液常规,部分脏器系数和病理检查为观察指标。结果中、高剂量组雌性和雄性动物试验期间体质量较对照组明显降低;高剂量组雌性动物血小板(Plt)降低,高剂量组雄性动物的白细胞(WBC)降低和血清尿素氮(BUN)升高。中、高剂量组动物部分脏器体比升高。中、高剂量的部分动物肾脏有不同程度病理改变。结论克菌丹在SD大鼠亚慢性经口试验中最大无作用剂量为:雌性36.0mg·kg-1·d-1,雄性30.9mg·kg-1·d-1。肾脏可能是克菌丹的靶器官。Objective To study the sub-chronic oral toxicity of captan,and to obtain its non-observed adverse effect level and targeted organs. Methods Sprague Dawley （SD） rats were divided into 4 groups： negative control, low, medium and high dose group, and orally treated to the basal diet with captan at the doses of 0 （control） ,36.0,124. 4,371.3 mg· kg^-1 · d^- 1 in female and 0,30. 9,94. 2,323. 6 mg·kg^-1 · d^-1 in male for 90 days. The general behaviors,body weight,bloed routine tests,12 blood biocheimical indexes,urine routine tests were tested. Organ coefficients and histopathological examination were observed. Results Compared with the control, body weights in female and male at medium and high dose groups decreased. PLT in female rats and WBC in male rats at high group decreased,while BUN in male at high group increased. Ratio of organ and body weight at medium and high group increased. Histopathological examinations revealed that some extent of changes were found in kidneys at medium and high dose groups. Conclusion The results showed that non-observed adverse effect level of eaptun in SD rats is 36. 0 mg· kg^-1 · d^-1 in female,and 30. 9 mg · kg^-1 · d^-1 in male,respectively. Kidney may be the targeted organ.

关 键 词：克菌丹 亚慢性经口毒性 最大无作用剂量 靶器官 

分 类 号：R994.3[医药卫生—毒理学]