Ca/GA对渗透胁迫下小麦种子萌发中蛋白质变化的调节  

Regulation of Ca/GA on Changes of Protein during Wheat Seed Germination under Osmotic Stress

作　　者：路苹[1] 汪沛洪[1] 山仑[2] 

机构地区：[1]西北农业大学植物生化研究室,陕西杨陵712100 [2]中国科学院西北水土保持研究所,陕西杨陵712100

出　　处：《作物学报》1998年第4期439-446,共8页Acta Agronomica Sinica

基　　金：国家攀登计划9219项目中"作物抗旱性的机理及其改善"专题资助

摘　　要：小麦萌发时,种子胚乳总蛋白含量逐渐下降,其中由于提供萌发所需氮化物球蛋白、醇溶蛋白和谷蛋白逐渐减少,合成数种启动种子萌发所需酶蛋白等的清蛋白逐渐增加。胚中蛋白质各组分随种子萌发过程均逐渐增加。渗透胁迫下,萌发种子胚乳清蛋白和胚中蛋白质各组分的增加,以及胚乳中球蛋白、醇溶蛋白和谷蛋白的降解均受到抑制。因此,种子萌发与其蛋白质变化密切相关。Ca处理在萌发后期才对种子蛋白质变化起促进作用,GA和Ca+GA处理在萌发后一直起促进作用。本实验结果表明,Ca/GA处理种子通过对萌发中蛋白质的调节,可减缓渗透胁迫对萌发造成的不良影响。对胚乳和胚中蛋白质变化的调节效果均是GA和Ca+GA处理优于Ca处理。Total protein content in wheat endosperm gradually declined during seed germination, but the contents of globulin, prolamin and glutelin decreased due to supplying nitrogen for germination, and the content of albumin gradually increased because of several kinds of enzymes synthesized for promoting seed germination. All protein composition gradually in creased in embryo. Under osmotic stress the increment of albumin in endosperm and all protein composition in embryo, and the degradation of globulin, prolamin and glutelin in endosperm were inhibited. Therefore wheat seed germination conditions were closely correlated with protein changes. Ca treatment was effective at the late stage of germination. Germination process was promoted using GA or Ca+GA treatment after seed germination. The Ca/GA treatments, pos sessed buffering effect for osmotic stress during seed germination via seed protein regulation. The GA or Ca+GA treatment was superior to Ca treatment.

关 键 词：小麦 种子萌发 蛋白质 钙 赤霉素 渗透胁迫 

分 类 号：S512.104[农业科学—作物学]