精子质量与形态学分析及其关系  

Analysis of sperm quality and morphology

作　　者：周其赵[1] 陈思梅[2] 冯春琼[3] 邹亚光[4] 孙德华[5] 李铁求[1] 李飞[1] 毛向明[1] 

机构地区：[1]南方医科大学南方医院泌尿外科,广州市510515 [2]南方医科大学南方医院生殖医学中心,广州市510515 [3]南方医科大学基因工程研究所,广州市510515 [4]南方医科大学南方医院口腔科,广州市510515 [5]南方医科大学南方医院检验科,广州市510515

出　　处：《实用医学杂志》2009年第14期2248-2250,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30772167);广州市科技计划项目(编号:2008J1-C131)

摘　　要：目的:探讨精子质量与形态学的临床应用价值及其关系。方法:248份精液标本按WHO标准进行精液常规分析,应用计算机辅助精液分析(CASA)分析精子密度、活力、活率以及动态参数;采用WHO推荐的Diff-Quik快速染色方法对精子形态进行染色;采用SPSS13.0软件进行统计学分析。结果:248份精子质量分析中,精子活力不足占74.6%,精子活力正常占25.4%,少精症占5.6%,少弱精症占4.8%,正常精液占24.6%;248份精子形态学分析中,形态正常精液占73.4%,畸形精子症占26.6%。精子活力和活率与形态正常精子百分率呈显著性正相关(r=0.52,r=0.53,P<0.01);各运动参数和精子密度也与形态正常精子百分率呈显著性正相关(P<0.01)。精子活力正常组形态正常精子百分率明显高于精子活力低下组(P<0.01)。结论:精子活力不足和畸形精子症是导致男性不育的主要原因,精子形态与精子活力、活率、密度和运动参数有密切关系,精子质量和形态学分析在临床男性不育的诊断中具有重要作用。Objective To investigate the clinical value of sperm quality and morphology and their association. Methods 248 semen samples were examined according to the WHO criteria. The sperm density, motility, viability, and dynamic parameters were analyzed by computer-aided sperm analysis （CASA） ; sperm morphology was detected using Diff- Quik staining recommended by WHO. The software SPSS 13.0 was used for statistical analysis. Results Poor sperm motility was found in 74.6% of all the semen samples, normal motility in 25.4%, Oligospermia in 5.6%, asthenospermia in 4.8%, and normal semen in 24.6%. The sperm morphology was normal in 73.4% of the samples and teratozoospermia occurred in 26.6%. Sperm motility and viability were positively related with the percetage of normal morphology （r = 0.52, r = 0.53,P 〈 0.01）, so were each dynamic parameter and sperm density （P 〈 0.01）. The percentage of normal sperm morphology was greater in the normal motility group than in the asthenospermia group （P〈 0.01 ）. Conclusions Asthenospermia and teratozoospermia are the major factors for male infertility. The sperm morphology is closely related to sperm motility, viability, density and dynamic parameters. The analysis of sperm quality and morphology plays an important role in the clinical diagnosis of male infertility.

关 键 词：不育 男(雄)性 精子形态 精子活力 精子密度 年龄 

分 类 号：R698.2[医药卫生—泌尿科学] R446.1[医药卫生—外科学]