棉花纤维长度主效QTLs的分子标记辅助选择及聚合效果研究  

Molecular Marker-assisted Selection and Pyramiding Breeding of Major QTLs for Cotton Fiber Length

作　　者：董章辉[1] 石玉真[1] 张建宏[1] 王淑芳[1] 李俊文[1] 刘爱英[1] 唐淑荣[1] 褚平[1] 袁有禄[1] 

机构地区：[1]中国农业科学院棉花研究所农业部棉花遗传改良重点实验室,河南安阳455000

出　　处：《棉花学报》2009年第4期279-283,共5页Cotton Science

基　　金：国家973项目(2004CB117306);863计划(2006AA100105550)

摘　　要：以2个品种(系)TG41和sGK156以及3个纤维品质优异的种质系7235、HS427-10和0-153为亲本,配制了(sGK156×HS427-10)×(0-153×7235)(、TG41×HS427-10)×(0-153×7235)和(sGK156×0-153)×(sGK156×HS427-10)3套组合的双交F1及F2群体,利用3个纤维长度不同QTLs相关的SSR标记进行辅助选择。结果,用3个标记分别进行单标记辅助选择时,有/无标记单株平均纤维长度之间的差异在3个群体的F1世代中都可以达到显著或极显著水平,并且在F2世代株行中也表现出一定差异,可以稳定表达。当用2个或3个标记同时进行聚合选择时,随着聚合到QTL个数的增多,单株平均纤维长度值增大,选择效果越来越好,但在不同群体中表现有差异。可见,用分子标记辅助选择的方法对棉花纤维长度进行改良是有效的,聚合多个QTLs时,可以达到更好的效果。有必要培育多个基因聚合并纯合的高代重组自交系材料,进一步研究多基因聚合的遗传效应。TG41 and sGK156, two commercial cotton cultivars （lines）, and HS427-10, 7235 and 0- 153, three elite fiber quality germplasm lines, were used as parents to develop three double-cross combinations, （sGK156 × HS427 10） × （0-153 × 7235）, （TG41× HS427 10） × （0-153× 7235） and （sGK156 × 0-153）× （sGK156 ×HS427-10）. Three SSR markers, NAU1043, MUSS497 and CM67, which were linked with QTLs of fiber length and derived from 7235 or 0-153, were used to study the effect of molecular marker assisted selection and pyramiding breeding in the three combinations, respectively. As a result, the fiber length showed significant differences between plants with and without marker in the three combinations. This indicated that the effects of the three QTLs for fiber length were stable in different genetic backgrounds and different generations. When selected the plants with two or three markers, the fiber length of plants with two or three markers were better than that of the plants with only one marker, and the fiber length was the best in the plants with all the three markers. In conclusion, it is effective to increase fiber length through molecular marker-assisted selection and the effect may be increased when more QTLs are pyramided in plants. It is necessary to develop stable recombinant inbred lines of advanced generations which pyramid several fiberrelated genes to study the effect for pyramiding more genes.

关 键 词：棉花 纤维长度 QTL 分子标记 育种 

分 类 号：S562[农业科学—作物学]