抗生素抑制农杆菌的效果及对条斑紫菜生长发育的影响  

The Effect of Antibiotics Inhibiting Agrobacterium and the Influence on the Growth and Development in Laver Porphyra yezoensis

作　　者：王萍[1] 成锋 滕亚娟[1] 李信书[1] 许星鸿[1] 

机构地区：[1]淮海工学院海洋学院江苏省海洋生物技术重点建设实验室,江苏连云港222005 [2]百奥生物技术(南通)有限公司,江苏南通226010

出　　处：《水产科学》2009年第7期405-408,共4页Fisheries Science

基　　金：江苏省海洋生物技术重点建设实验室开放课题(2005HS011);淮海工学院引进人才科研启动基金资助项目(KK01073)

摘　　要：用头孢霉素和卡那霉素抑制农杆菌(LBA4404和EHA105)生长,同时测定在液体培养条件下条斑紫菜组织块对2种抗生素的敏感性,以期找到适合用于农杆菌介导条斑紫菜遗传转化的脱菌剂及农杆菌转化用于条斑紫菜的可行性。试验结果表明,2种抗生素对2株农杆菌菌株有较强的抑制作用,对条斑紫菜叶状体组织块的生长作用效果不同,头孢霉素≥300 mg/L抑制叶状体组织块长度的增加,而卡那霉素≥400 mg/L对组织块的增长有一定的促进作用。头孢霉素对条斑紫菜的发育抑制作用较卡那霉素更大。在培养液中添加头孢霉素≥300 mg/L或卡那霉素质量浓度≥600 mg/L组织块不能发育形成丝状体。头孢霉素和卡那霉素可作为农杆菌介导条斑紫菜遗传转化的抑菌剂,使用较适宜的质量浓度分别为200 mg/L和400 mg/L。Cefotaxime and kanamycin sulfate were used to inhibit the growth of Agrobacterium （LBA4404 and EHA105）and the sensitivity of thallus of laver Porphyra yezoensis cultured in the liquid media to two kinds of antibiotics were measured in order to investigate the bacteria-elimination agent which mediated genetic transformation in the laver via Agrobacterium-medium and to verify the feasibility. The results showed that the two kinds of antibiotics had a strong inhibition on the growth of Agrobacterium and a dif- ferent influence on the growth of thalli. Cefotaximes above 300 mg/L inhibited the length of thallus and kanamycin sulfate above 400 mg/L promoted the growth of thallus. The effect of cefotaximes on inhibiring the development of the thallus in the laver was bigger than that of kanamycin. Conchocelis did not develop and form in media with cefotaximes above 300 mg/L or kanamycin sulfate above 600 mg/L. Cefotaximes and kanamycin sulfate were used for genetic transformation of the laver as an inhibitor via Agrobacterium-medium. The appropriate concentration of cefotaximes and kanamycin sulfate was 200 mg/ L and 400 mg/L,respectively.

关 键 词：条斑紫菜 农杆菌 抗生素 生长与发育 

分 类 号：S968.431[农业科学—水产养殖]