Influence of CDK1 and CDK2 siRNA interference on tumor cell cycle and cell apoptosis  

CDK1、CDK2 siRNA干扰对肿瘤细胞凋亡和细胞周期的影响(英文)

作　　者：Hui Xiao Wanjun Gong Jingpeng Cao Xiaolan Li Deding Tao Junbo Hu Jianping Gong 

机构地区：[1]Cancer Research Institute, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China

出　　处：《The Chinese-German Journal of Clinical Oncology》2009年第7期371-374,共4页中德临床肿瘤学杂志（英文版）

基　　金：Supported by grants from the National Basic Research Program of China (No. 2004CB518705);National Natural Sciences Foundation of China (No.30570908)

摘　　要：Objective： We investigated the influence of CDK1 and CDK2 expression inhibited by cotransfection of CDK1 and CDK2 siRNA on cell cycle and apoptosis, explored the exact role of cell cycle master regulator in tumor cell apoptosis process. Methods： The siRNA targeting the CDK1 and CDK2 genes were synthesized and simultaneously cotransfected into Hela cells by lipofectamine 2000.48 or 60 h after the cotransfection, CDK1 and CDK2 protein expressions were examined by Western blot. Cell cycle distribution was analyzed by flow cytometry. Cell apoptosis was detected by the Annexin V/PI method. The changes of the transfected cell morphological under a microscope after Wright-Giemsa Staining were studied. Results： CDK1 and CDK2 protein expression was decreased at 48 or 60 h after cotransfection. The accumulation of the G2/M and S phase population in cell cycle of the cotransfected cells at 48 or 60 h after transfection was enhanced obviously compared with control. The ratio of apoptotic cell of cotransfected cells at 48 or 60 h after transfection was increased significantly compared with control. More binucleate or multinucleate ceJls among cotransfected cells were observed under the microscope. Conclusion： The decreased expression of CDK1 and CDK2 by cotransfection of CDK1 and CDK2 siRNA not only leads to tumor cell cycle arrest in S phase and G2/M phase, but also induces tumor cell apoptosis.

关 键 词：CDK1 CDK2 cotransfection of siRNA cell cycle arrest apoptosis 

分 类 号：Q279[生物学—细胞生物学] Q2